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Skin lipids from Saudi Arabian birds
Joint Authors
Champagne, Alex M.
Shobrak, Muhammad
Arif, Ibrahim Abd al-Wahid
Williams, Joseph B.
Khan, Hasib A.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 2 (30 Apr. 2014), pp.173-177, 5 p.
Publisher
Publication Date
2014-04-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Abstract EN
Skin lipids play an important role in the regulation of cutaneous water loss (CWL).
Earlier studies have shown that Saudi desert birds exhibit a tendency of reduced CWL than birds from temperate environment due to adaptive changes in composition of their skin lipids.
In this study, we used thin-layer chromatography (TLC) for separation and detection of nonpolar and polar lipids from the skin of six bird species including sooty gull, brown booby, house sparrow, Arabian waxbill, sand partridge, and laughing dove.
The lipids were separated and detected on Silica gel G coated TLC plates and quantified by using densitometric image analysis.
Rf values of the non-polar lipids were as follows: cholesterol (0.29), free fatty acids (0.58), triacylglycerol (0.69), fatty acids methyl esters (0.84) and cholesterol ester (0.97).
Rf values for the polar lipids were: cerebroside (0.42), ceramide (0.55) and cholesterol (0.73).
The results showed the abundance of fatty acids methyl esters (47.75–60.46%) followed by triacylglycerol (12.69–24.14%).
The remaining lipid compositions were as follows: cholesterol (4.09–13.18%), ceramide (2.18–13.27%), and cerebroside (2.53–12.81%).
In conclusion, our findings showed that TLC is a simple and sensitive method for the separation and quantification of skin lipids.
We also reported a new protocol for lipid extraction using the zirconia beads for efficient disruption of skin tissues.
This study will help us better understand the role of skin lipids in adaptive physiology towards adverse climatic conditions.
American Psychological Association (APA)
Khan, Hasib A.& Arif, Ibrahim Abd al-Wahid& Williams, Joseph B.& Champagne, Alex M.& Shobrak, Muhammad. 2014. Skin lipids from Saudi Arabian birds. Saudi Journal of Biological Sciences،Vol. 21, no. 2, pp.173-177.
https://search.emarefa.net/detail/BIM-766967
Modern Language Association (MLA)
Khan, Hasib A.…[et al.]. Skin lipids from Saudi Arabian birds. Saudi Journal of Biological Sciences Vol. 21, no. 2 (Apr. 2014), pp.173-177.
https://search.emarefa.net/detail/BIM-766967
American Medical Association (AMA)
Khan, Hasib A.& Arif, Ibrahim Abd al-Wahid& Williams, Joseph B.& Champagne, Alex M.& Shobrak, Muhammad. Skin lipids from Saudi Arabian birds. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 2, pp.173-177.
https://search.emarefa.net/detail/BIM-766967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 176-177
Record ID
BIM-766967