Effect of different low protein diets supplemented with vitamin E, nigella sativa seeds, and green tea on renal functions among rats

Abstract EN

The present study was conducted to investigate the influence of vitamin E, Nigella Sativa seeds, green tea leaves and their combinations on renal functions of rats fed L-arginine diet.

The two main experimental groups; include Group 1 (n=6 rats) used as a negative control group fed on basal diet and group 2 (n= 54 rats) fed on L-arginine diet (2% L-arginine) along the study period.

After two week of feeding Larginine diet, this group was divided into nine subgroups (6 rats each).

Subgroup (1) was fed on L-arginine diet, as a control positive group; Subgroups (2 and 3) were fed on low protein diets (10% protein) supplemented with 100 and 200 mg vitamin E/ 100g diet, respectively.

Subgroups (4 and 5) were fed on low protein diets supplemented with 2.5% and 5% Nigella Sativa seed/100g diet, respectively.

Subgroups (6 and 7) were fed on low protein diets supplemented with 2.5% and 5% green tea/100g diet, respectively.

Subgroups (8 and 9) were fed on low protein diets supplemented with 100 mg vitamin E, 2.5% Nigella Sativa seed and 2.5% green tea & 200 mg vitamin E, 5% Nigella Sativa seed and 5% green tea/100g diet, respectively.

Food intake increased significantly among experimental groups fed 5% Nigella Sativa seeds and the group fed the higher concentration of mixture supplements, on the other hand body weight gain% increased significantly in all treated groups, except for group fed 5% green tea.

All experimental groups with the two concentration from (vitamin E, Nigella Sativa seeds, green tea and their combinations) resulted in improvement in all parameters including (kidney functions, liver enzymes, lipid profile, glutathione peroxidase, superoxide dismutase and catalase), especially the group which fed the higher concentration of supplements.

In conclusion, vitamin E, Nigella Sativa seeds, green tea leaves and their combination improved the nutritional and biological status of rats renal insufficiency