Spectrophotometric determination of cefotaxime via diazotization reaction in pure and pharmaceutical samples
Joint Authors
Muhammad, Najah Muhammad
Abbas, Sumayyah Muhammad
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 30, Issue 2 (31 Aug. 2017), pp.151-165, 15 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2017-08-31
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Topics
Abstract EN
An accurate and sensitive spectrophotometric method has been developed for the determination of cefotaxime (CEF) in pure and pharmaceutical samples.
The suggested method depended on the coupling reaction between diazotized cefotaxime and 3,5-dimethyl phenol (3,5-DMPH) in basic medium to form light orange, water soluble dye, that is stable and has a maximum absorbance at 497nm.
The calibration graph was liner over the concentration range (1-70) μg.mL-1 with LOD of 0.750 μg.mL-1 and LOQ of.
2.740 μg.
mL-1, sandal sensitivity of 0.0526 μg.
cm-2 .
molar absorptivity 11328 Lmol-1 cm-1 .
The stoichiometry composition was found by Jobs and mole ratio methods to the formation dye (1:1).
The suggested method has been applied successfully to the determination of CEF in pure and pharmaceutical synthetic sample.
American Psychological Association (APA)
Abbas, Sumayyah Muhammad& Muhammad, Najah Muhammad. 2017. Spectrophotometric determination of cefotaxime via diazotization reaction in pure and pharmaceutical samples. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 30, no. 2, pp.151-165.
https://search.emarefa.net/detail/BIM-768285
Modern Language Association (MLA)
Abbas, Sumayyah Muhammad& Muhammad, Najah Muhammad. Spectrophotometric determination of cefotaxime via diazotization reaction in pure and pharmaceutical samples. Ibn al-Haitham Journal for Pure and Applied Science Vol. 30, no. 2 (2017), pp.151-165.
https://search.emarefa.net/detail/BIM-768285
American Medical Association (AMA)
Abbas, Sumayyah Muhammad& Muhammad, Najah Muhammad. Spectrophotometric determination of cefotaxime via diazotization reaction in pure and pharmaceutical samples. Ibn al-Haitham Journal for Pure and Applied Science. 2017. Vol. 30, no. 2, pp.151-165.
https://search.emarefa.net/detail/BIM-768285
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 157-165
Record ID
BIM-768285
