Molecular detection of enterococcus surface protein (Esp)‎ gene in E.faecalis and E.faecium and their role in biofilm formation

Joint Authors

al-Hashimi, Ashwaq B.
al-Kazimi, Isra Muhammad Safi
al-Halabi, Aya H.
al-Ujayli, Imad

Source

Iraqi Journal of Biotechnology

Issue

Vol. 16, Issue 1 (31 Mar. 2017), pp.80-91, 12 p.

Publisher

University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies

Publication Date

2017-03-31

Country of Publication

Iraq

No. of Pages

12

Main Subjects

Biology
Medicine

Topics

Abstract EN

Total of (104) urine samples were collected from patients suffering from urinary tract infection with different age groups from five hospitals in Baghdad (Ibn- Albalady, Al Yarmouk, Medical city, Baghdad hospital and Al-Kandy) from the period of the beginning of September to the end of December 2015.

All samples were examined by traditional methods based on cultural characteristics, biochemical test and API 20 strep.

The results showed the revealed of 50 isolates to Enterococcus and this confirmed by polymerase chain reaction technique based on amplification of species specific genes.

PCR were performed for E.faecalis and E.faecium in order to confirm the presence of Esp gene which coding for Enterococcus surface protein using specific primer for gene, the results showed Enterococcus contain a proportion of 54% of Esp.

Biofilm production was detected in E.faecalis and E.faecium by use two methods: Congo red agar method and microtiter plate method, our results showed 22(44%) of Enterococcus isolates was strong biofilm production, 25(50%)as moderate and 3(6%) as week biofilm production.

By use Congo red method, in microtiter plate method results showed that 20(40%) of bacterial isolates was detected as strong, 26(52%) as moderate and 4(8%) as week biofilm production.

American Psychological Association (APA)

al-Halabi, Aya H.& al-Hashimi, Ashwaq B.& al-Kazimi, Isra Muhammad Safi& al-Ujayli, Imad. 2017. Molecular detection of enterococcus surface protein (Esp) gene in E.faecalis and E.faecium and their role in biofilm formation. Iraqi Journal of Biotechnology،Vol. 16, no. 1, pp.80-91.
https://search.emarefa.net/detail/BIM-769155

Modern Language Association (MLA)

al-Halabi, Aya H.…[et al.]. Molecular detection of enterococcus surface protein (Esp) gene in E.faecalis and E.faecium and their role in biofilm formation. Iraqi Journal of Biotechnology Vol. 16, no. 1 (2017), pp.80-91.
https://search.emarefa.net/detail/BIM-769155

American Medical Association (AMA)

al-Halabi, Aya H.& al-Hashimi, Ashwaq B.& al-Kazimi, Isra Muhammad Safi& al-Ujayli, Imad. Molecular detection of enterococcus surface protein (Esp) gene in E.faecalis and E.faecium and their role in biofilm formation. Iraqi Journal of Biotechnology. 2017. Vol. 16, no. 1, pp.80-91.
https://search.emarefa.net/detail/BIM-769155

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 89-91

Record ID

BIM-769155