Determination of the infective strain of hydatid cyst in Iraqi cattle by using co1 gene
Other Title(s)
تحديد السلالة المسببة للأكياس المائية في الأبقار العراقية باستخدام جين 1 co
Author
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 48, Issue 2 (30 Apr. 2017), pp.644-649, 6 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2017-04-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Hydatid Cysts were obtained from liver, lungs, spleen, heart, and peritoneal cavity of 15 cows, from different Iraqi regions between December 2014 and October 2015.
Hydatid cysts (protoscoleces) were used for mitochondrial DNA extraction by using mechanical grinder, and the purification of mtDNA was done by (promega kit, USA).
"The mitochondrial cytochrome c oxidase subunit 1 (CO1) gene" was used as target for "polymerase chain reaction (PCR)" which successfully amplified the targeted this gene with 450 bp.
The PCR products were purified and partial sequences were determine.
The obtained sequences were aligned with the corresponding region of co1 gene in the Gene Bank nucleotide database to confirm the infection with hydatid cyst sheep strain (G1) in Iraq.
The amplified CO1 targeted region was analyzed to obtain the phylogenetic tree.
G1 genotype was the most common strain and the actual source of infection of Iraqi's cattle.
All of 15 samples were G1 strain (sheep strain) according to the partial sequences of (CO1) genes.
American Psychological Association (APA)
Muhaidi, Muhammad Jubayr. 2017. Determination of the infective strain of hydatid cyst in Iraqi cattle by using co1 gene. The Iraqi Journal of Agricultural Science،Vol. 48, no. 2, pp.644-649.
https://search.emarefa.net/detail/BIM-770463
Modern Language Association (MLA)
Muhaidi, Muhammad Jubayr. Determination of the infective strain of hydatid cyst in Iraqi cattle by using co1 gene. The Iraqi Journal of Agricultural Science Vol. 48, no. 2 (2017), pp.644-649.
https://search.emarefa.net/detail/BIM-770463
American Medical Association (AMA)
Muhaidi, Muhammad Jubayr. Determination of the infective strain of hydatid cyst in Iraqi cattle by using co1 gene. The Iraqi Journal of Agricultural Science. 2017. Vol. 48, no. 2, pp.644-649.
https://search.emarefa.net/detail/BIM-770463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 648-649
Record ID
BIM-770463