Detection of metallo-β-lactamase producing pseudomonas aeruginosa in clinical isolates from hospitals in Erbil city, Iraqi Kurdistan

Abstract EN

Multidrug resistance (MDR) became a great concern in P.

aeruginosa has appeared as issue of great concern with the emergence of Metallo-β-lactamases (MBL)-producing isolates, which showed to be the primary resistance mechanism in carbapenem-resistant P.

aeruginosa.

The aim of the present study was to detect MBL-producing P.

aeruginosa isolates from patients and evaluating antibiotic guide for clinicians in prescribing accurate antibiotic and to further controlling hospital infections.

A total of 98 consecutive isolates of P.

aeruginosa causing infections were isolated from various clinical samples.

We applied Vitek-2 automated system as a panel of antimicrobial agents.

Imipenem-EDTA combined disk test (CDT) and modified Hodge test (MHT) for phenotypic detection of MBL-producing isolates were followed.

Out of 98 isolates of P.aeruginosa, only 72 isolates (73%) showed IMP-resistance and 26 (26%) were sensitive to imipenem.

Around 63 (87.5%) of the IMP-resistant isolates were able to produce MBLs as determined by CDT, while 30 (41.6%) showed positive results in MHT.

The resistance to tested antibiotics was significantly higher (P <0.001) with MBL producing P.

aeruginosa isolates compared to non-MBL producers.

The prevalence of MDR to the antibiotics among MBL-producing P.

aeruginosa isolates was established.

CDT is a reliable screening test for the detection of MBL producer