Immune response of Balb C mice against genetic vaccination with Leismania major GP63 gene (lmajgp63)
Other Title(s)
الاستجابة المناعية لفئران Balb c ضد التطعيم الجيني بجين Liesh mania major gp63 (lmajgp63)
Joint Authors
Abbas, Wamidh Hashim
Ali, Salman Abd al-Halim
Mahdi, Nazim Kazim
Source
Basrah Journal of Veterinary Research
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.13-34, 22 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
22
Main Subjects
Abstract EN
Leishmania major glycoprotein 63 (lmajgp63) gene was used in this study as DNA vaccine candidates.
Gene was inserted into VR1012 plasmid by using standard molecular biology protocols, resulting in preparation of lmajgp63/VR1012 plasmid.
Vaccine either used as naked or gold particles coated DNA vaccine in immunization of females Balb/c mice.
Animals were immunized at week 0, 2 weeks and 6 weeks.
Dermojet needle free injector had been used to deliver gold particles coated DNA vaccine intradermally (I/D) while ordinary needle injection was used to deliver naked vaccine intramuscularly (I/M).
Immune response for each vaccinated group were detected, two weeks after the third administration of the vaccines, by estimation of serum concentration of IL-2, IL-4, IL-10 and INF-γ, as well as anti-soluble Leismania antigen (anti-SLA) IgG titer, by ELISA test.
The results demonstrated the effectiveness of DNA vaccines in induction immune response comparing to control groups (P<0.05).
The highest serum concentrations of IL-2, INF-γ and anti-SLA IgG OD value observed in mice group which immunized with gold particles coated vaccine injected I/D (182.10 pg/ml, 1699.20 pg/ml and 0.6101 respectively), while the lowest titer was observed in group vaccinated with naked plasmids injected I/M (103.60 pg/ml, 1183.20 pg/ml and 0.3395 respectively).
On the other hand group treated with naked plasmid I/M shows the highest titer of IL-4 and IL-10 concentration (85.30 pg/ml and 53.10 pg/ml respectively), while the lowest titer observed in group injected I/D by gold coated plasmid (50.90 pg/ml and 41.88 pg/ml respectively).
The results also demonstrated that I/D injection of gold particles coated DNA vaccine induced the highest Th1/Th2 response [IL-2/IL-10 (4.348) and INF-γ/IL-4 (33.383)], while I/M injection of naked vaccine gave the lowest results [IL-2/IL-10 (1.951) and INF-γ/IL-4 (13.879)].
American Psychological Association (APA)
Abbas, Wamidh Hashim& Mahdi, Nazim Kazim& Ali, Salman Abd al-Halim. 2016. Immune response of Balb C mice against genetic vaccination with Leismania major GP63 gene (lmajgp63). Basrah Journal of Veterinary Research،Vol. 15, no. 1, pp.13-34.
https://search.emarefa.net/detail/BIM-771153
Modern Language Association (MLA)
Abbas, Wamidh Hashim…[et al.]. Immune response of Balb C mice against genetic vaccination with Leismania major GP63 gene (lmajgp63). Basrah Journal of Veterinary Research Vol. 15, no. 1 (Jun. 2016), pp.13-34.
https://search.emarefa.net/detail/BIM-771153
American Medical Association (AMA)
Abbas, Wamidh Hashim& Mahdi, Nazim Kazim& Ali, Salman Abd al-Halim. Immune response of Balb C mice against genetic vaccination with Leismania major GP63 gene (lmajgp63). Basrah Journal of Veterinary Research. 2016. Vol. 15, no. 1, pp.13-34.
https://search.emarefa.net/detail/BIM-771153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 31-34
Record ID
BIM-771153