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Diagnosis of bovine Anaplasma Marginale in north western Libya using serology and blood film examination : a comparative study
Other Title(s)
تشخيص إصابة الأبقار بجرثومة Anaplasma marginale في شمال غرب ليبيا باستخدام فحص المسحات الدموية و الفحوصات المصلية : دراسة مقارنة
Joint Authors
al-Basam, Layla Subhi
al-Gharib, Sulayman O.
al-Attar, Sayyid R.
Iazim, Abd al-Nasir
Abd al-Salam, Umran Imhammad
Source
Basrah Journal of Veterinary Research
Issue
Vol. 15, Issue 1 (30 Jun. 2016), pp.119-131, 13 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
Anaplasmamarginale (A.
marginale) is an obligate intra-erythrocytic rickettsia;it is the cause of anaplasmosis, an important tick-borne disease of cattle.
Recovered and vaccinated cattle in endemic areas are apparently normal but remain persistently infected and serve as reservoirs for the parasite.This study intended to detect A.
marginale in infected and apparently healthy subclinically infected cattle in North Western Libya.
During the period extended from March-2006 till September-2007, blood samples and blood smears were collected from totally 119 adult cow (group – I).
These cows were raised at some governmental and private farms in Tripoli, Al-Zawiya and Imssallata districts.
Blood smears were stained with May-Grunewald- Giemsa stain and examined under Light microscope to detect the presence of intraerythrocytic bacteria.
Indirect- ELISA (IELISA) using a 19 KD A.
marginale recombinant antigen was used to detect serologically positive reactors.During the study period, 20 cases of acute anaplasmosis were diagnosed in these farms (Group-II); where, three cows died and two aborted.
The Seroprevalence for A.
marginale by IELISAwas 64% and 100% in group I and II, respectively.
Stained thin blood smears failed to detect infective RBCs in group I, however, variable degrees of parasitaemia were detected in group II.
In conclusion, this study approved that serological test (IELISA) was more reliable than direct microscopic examination of stained blood smear in detection of chronic persistent anaplasma-infected cows in endemic areas.
American Psychological Association (APA)
al-Basam, Layla Subhi& al-Gharib, Sulayman O.& al-Attar, Sayyid R.& Iazim, Abd al-Nasir& Abd al-Salam, Umran Imhammad. 2016. Diagnosis of bovine Anaplasma Marginale in north western Libya using serology and blood film examination : a comparative study. Basrah Journal of Veterinary Research،Vol. 15, no. 1, pp.119-131.
https://search.emarefa.net/detail/BIM-771162
Modern Language Association (MLA)
al-Basam, Layla Subhi…[et al.]. Diagnosis of bovine Anaplasma Marginale in north western Libya using serology and blood film examination : a comparative study. Basrah Journal of Veterinary Research Vol. 15, no. 1 (Jun. 2016), pp.119-131.
https://search.emarefa.net/detail/BIM-771162
American Medical Association (AMA)
al-Basam, Layla Subhi& al-Gharib, Sulayman O.& al-Attar, Sayyid R.& Iazim, Abd al-Nasir& Abd al-Salam, Umran Imhammad. Diagnosis of bovine Anaplasma Marginale in north western Libya using serology and blood film examination : a comparative study. Basrah Journal of Veterinary Research. 2016. Vol. 15, no. 1, pp.119-131.
https://search.emarefa.net/detail/BIM-771162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 127-131
Record ID
BIM-771162