Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in al-Muthanna province-Iraq
Other Title(s)
التعبير الجزيئي لكال الجينين CIT و الجين FOX في بكتريا الكليبسيلا المعزولة من مستشفى الحسين التعليمي في محافظة المثنى
Author
al-Karawi, Abir Muhammad Ali Jasim
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 7, Issue 2 (31 Dec. 2016), pp.33-47, 15 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Abstract EN
klebsiella is the most common pathogen responsible for acquired infections in the hospitals and because of the extensive ability to produce β-lactamases made this bacteria resistance to many antimicrobial agents.
This study was done to determine Klebsiella pneumoniae isolates containing both blaFOX and blaCIT by multiplex polymerase chain reaction .
Out of fifty one Klebsiella isolates , 31 (60.8%) K.
pneumoniae isolates were resistant to cefoxitin by disk diffusion method .
The production of molecular class C β-lactamase tested through modified three dimension method in all cefox resistance isolates and observed 14 (45.2%) isolates were producing for AmpC β-lactamase .
The cefoxitin resistance isolates further screened by AmpC disk method and the result was 11 (35.5%)isolates were producing for AmpC β-lactamase .
Multiplex PCR technique showed 8/31 (25.8%) K.
pneumoniae isolates possess AmpC β- lactamase gene as well as this technique also revealed that FOX gene was exists in (37.5%) and CIT gene (62.5%)of the isolates .
Keywords: Klebsiella pneumoniae, AmpC, CIT , FOX and PCR.
American Psychological Association (APA)
al-Karawi, Abir Muhammad Ali Jasim. 2016. Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in al-Muthanna province-Iraq. Kufa Journal for Veterinary Medical Sciences،Vol. 7, no. 2, pp.33-47.
https://search.emarefa.net/detail/BIM-771353
Modern Language Association (MLA)
al-Karawi, Abir Muhammad Ali Jasim. Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in al-Muthanna province-Iraq. Kufa Journal for Veterinary Medical Sciences Vol. 7, no. 2 (Dec. 2016), pp.33-47.
https://search.emarefa.net/detail/BIM-771353
American Medical Association (AMA)
al-Karawi, Abir Muhammad Ali Jasim. Molecular expression of blaCIT and blaFOX in Klebsiella spp. isolates from Al-Hussein teaching hospital in al-Muthanna province-Iraq. Kufa Journal for Veterinary Medical Sciences. 2016. Vol. 7, no. 2, pp.33-47.
https://search.emarefa.net/detail/BIM-771353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 45-47
Record ID
BIM-771353