Isolation and identification of salmonella enteritidis using bacteriological and molecular technique from calves with diarrhea in Diwanihyia city
Other Title(s)
عزل و تشخيص salmonella entritidis من العجول المصابة بالإسهال باستخدام طرق بكتريولوجية و جزيئية في مدينة الديوانية
Joint Authors
Said, Asil Abd al-Rida
Qaid, Khitam
Klaif, Siba Falah
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 7, Issue 2 (31 Dec. 2016), pp.71-78, 8 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Salmonella enteritidis is an important foodborne pathogen that affects the human health by conception animal products and it was considered as a worldwide public health hazard.
This study was conduct to investigated the prevalence of S.
enteritidis, in calves with diarrhea in farm of AL- Diwanihyia city basis of molecular properties of this bacterium .
A total of 91 samples were collected from December 2013 to May 2014, in different farms in the al Diwanihyia city .
the pathogen was isolated by using microbiological and biochemical tests.
the DNA extraction was done by genomic DNA kit according to the manufacturer’s instruction (USA) and PCR was performed via the specific primers of SefA- -F and SeA-R of the SefA gene.
Amplified fragments of the 210 bp were observed in 12 of the total 91 stool samples isolates.
This study recommends that the identification of these pathogens by PCR technique can be replaced with traditional bacteriological tools.
The PCR method is a rapid approach for recognizing and identifying the S.
enteritidis infections in diarrheic calves.
American Psychological Association (APA)
Said, Asil Abd al-Rida& Qaid, Khitam& Klaif, Siba Falah. 2016. Isolation and identification of salmonella enteritidis using bacteriological and molecular technique from calves with diarrhea in Diwanihyia city. Kufa Journal for Veterinary Medical Sciences،Vol. 7, no. 2, pp.71-78.
https://search.emarefa.net/detail/BIM-771364
Modern Language Association (MLA)
Said, Asil Abd al-Rida…[et al.]. Isolation and identification of salmonella enteritidis using bacteriological and molecular technique from calves with diarrhea in Diwanihyia city. Kufa Journal for Veterinary Medical Sciences Vol. 7, no. 2 (Dec. 2016), pp.71-78.
https://search.emarefa.net/detail/BIM-771364
American Medical Association (AMA)
Said, Asil Abd al-Rida& Qaid, Khitam& Klaif, Siba Falah. Isolation and identification of salmonella enteritidis using bacteriological and molecular technique from calves with diarrhea in Diwanihyia city. Kufa Journal for Veterinary Medical Sciences. 2016. Vol. 7, no. 2, pp.71-78.
https://search.emarefa.net/detail/BIM-771364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 76-78
Record ID
BIM-771364