Molecular detection of coxiella burnetii in human and sheep in al-Diwaniyah province by real time-PCR
Other Title(s)
الكشف الجزيئي للالكوكسيلا البيورنتية في الإنسان و الأغنام في محافظة الديوانية باستخدام تقنية تفاعل سلسلة البلمرة في الوقت الحقيقي
Joint Authors
al-Hamdawi, Khatam Qayid Myia
Wadai, Ghusun Muhammad Ali
Ayiz, Numan Naji
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 7, Issue 2 (31 Dec. 2016), pp.193-203, 11 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
Background: Iraq is agricultural country, but the important aspect of livestock shown by most of the studies conducted in previous years, which dealt with Q fever infection in animals ( cattle, sheep and goat ) strikingly.
Q fever it is a worldwide zoonotic disease caused by Coxiella burentii .
Material and methods: One hundred and five (105), human samples used in the study were (50) (22 sputum/28 bronchial-alveolar lavage) collected from Teaching Hospital and Center Pulmonology and Respiratory Specialist in Al-Diwaniyah.
Collected sheep samples were (55) Sample (28 nasopharyngeal swabs and 27 bronchial fluids) from the slaughterhouse of Al-Diwaniya during January – august 2016.
All samples were collected from peoples & sheep that suffer from clear respiratory signs.
By using Sybr Green Real Time- PCR technique the samples tested directly.
Results: showed that the infection incidence in humans (16%) and in sheep (38.181%), the study proved that there were a significant differences at the site of infection in the respiratory tract (URT& LRT) for humans and sheep alike, as well as in terms of age groups and gender.
Conclusions: Q fever is a serious disease circulating between peoples in Al-Diwaniyah city and clearly defined risk groups: people with respiratory infection, heart and vascular disease, immunosuppressed patients and distributed in sheep that have respiratory disorder.
Real Time –PCR is a potential tool to diagnosis of C.
burnetii in humans and animals, because of its rapid and sensitive detection.
American Psychological Association (APA)
al-Hamdawi, Khatam Qayid Myia& Ayiz, Numan Naji& Wadai, Ghusun Muhammad Ali. 2016. Molecular detection of coxiella burnetii in human and sheep in al-Diwaniyah province by real time-PCR. Kufa Journal for Veterinary Medical Sciences،Vol. 7, no. 2, pp.193-203.
https://search.emarefa.net/detail/BIM-771410
Modern Language Association (MLA)
al-Hamdawi, Khatam Qayid Myia…[et al.]. Molecular detection of coxiella burnetii in human and sheep in al-Diwaniyah province by real time-PCR. Kufa Journal for Veterinary Medical Sciences Vol. 7, no. 2 (Dec. 2016), pp.193-203.
https://search.emarefa.net/detail/BIM-771410
American Medical Association (AMA)
al-Hamdawi, Khatam Qayid Myia& Ayiz, Numan Naji& Wadai, Ghusun Muhammad Ali. Molecular detection of coxiella burnetii in human and sheep in al-Diwaniyah province by real time-PCR. Kufa Journal for Veterinary Medical Sciences. 2016. Vol. 7, no. 2, pp.193-203.
https://search.emarefa.net/detail/BIM-771410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 200-203
Record ID
BIM-771410