Identification, expression and localization of the Ul45 protein of equine herpes virus type 1
Other Title(s)
تحديات و تعبير و توطين البروتين رقم 15 لفيروس هريس الخيول نوع 1
Joint Authors
Nayil, Muhammad
Qasim, Sami
al-Bajuri, Jabr
Abd al-Qadir, Ali
Dawud, al-Sayyid
Kamal, Tarik
Fukushi, Hideto
al-Khalifah, Nura Faysal
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 13, Issue 1 (30 Jun. 2015), pp.35-45, 11 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2015-06-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
UL45 was one of the tegument proteins that have been identified in both EHV-1 and EHV-4, it was recorded that ORF 15 EHV-1was homologues to UL45 (HSV).
Until now, the function of UL45 protein in cell culture and animal models is poorly understood.
To investigate the function of the UL45 protein, we expressed the UL45 gene in prokaryotic expression vector pGEX-6P-1 to express UL45 protein and used the resulting protein to raise guinea pig antiserum.
In western blotting experiments, the antiserum directed against the Nterminal of EHV-1 UL45 protein specifically recognized a protein with molecular weight ranging from 30 to 60 kDa in the infected cells lysates.
The immunofluorescence assay also showed that EHV-1 ORF15 antiserum was able to probe the UL45 protein which is localized mainly in the cytoplasm associating with the membrane of the cells infected with EHV-1 Ab4p.
American Psychological Association (APA)
Qasim, Sami& al-Bajuri, Jabr& Abd al-Qadir, Ali& Fukushi, Hideto& al-Khalifah, Nura Faysal& Nayil, Muhammad…[et al.]. 2015. Identification, expression and localization of the Ul45 protein of equine herpes virus type 1. Kafr El-Sheikh Veterinary Medical Journal،Vol. 13, no. 1, pp.35-45.
https://search.emarefa.net/detail/BIM-774152
Modern Language Association (MLA)
Qasim, Sami…[et al.]. Identification, expression and localization of the Ul45 protein of equine herpes virus type 1. Kafr El-Sheikh Veterinary Medical Journal Vol. 13, no. 1 (2015), pp.35-45.
https://search.emarefa.net/detail/BIM-774152
American Medical Association (AMA)
Qasim, Sami& al-Bajuri, Jabr& Abd al-Qadir, Ali& Fukushi, Hideto& al-Khalifah, Nura Faysal& Nayil, Muhammad…[et al.]. Identification, expression and localization of the Ul45 protein of equine herpes virus type 1. Kafr El-Sheikh Veterinary Medical Journal. 2015. Vol. 13, no. 1, pp.35-45.
https://search.emarefa.net/detail/BIM-774152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 52-54
Record ID
BIM-774152