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Isolation and identification of methicillin resistant staphylococcus aureus (MRSA) from poultry meat and poultry products
Other Title(s)
عزل و تصنيف الميكروب المكور العنقودي الذهبي المقاوم للميزيسلن من لحوم و منتجات الدواجن
Joint Authors
Hilmi, Salwa Muhammad
Abu Rumaylah, Itab Muhammad
Ramadan, Ahmad Hijazi
Nasif, Suad Abd al-Aziz Abd al-Wanis
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 13, Issue 1 (30 Jun. 2015), pp.57-69, 13 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2015-06-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
A total of 500 different samples were collected from poultry meat, poultry products and their human contacts in abattoirs from different Egyptian Governorates (Damietta,Dakahlia,Giza and Cairo) in 2012 and 2013.
the Samples were tested for presence ofS.
aureusby isolation and biochemical identification, the results revealed that coagulase positive S.
aureuswas present in83 samples (61.03%).The isolated strains of coagulase positive S.aureus were tested for antimicrobial sensitivity.
Most strains were resistant to penicillin and ampicillin, less resistance was appeared to amoxicillin, oxacillin, Methicillin, and ceftriaxone, while all strains were sensitive to, amoxicillin clavulinic acid and vancomycin.
Using PCR technique, amplification of 310 bp fragment of mecA gene from the extracted DNA of all isolated coagulase positive S.aureus strains isolated from different chicken samples and human contacts resulted in 5 samples from muscles and skin were positive, Using sequencing technique tomec A gene in three positive strains, the Phylogenetic analysis of mecA gene of these isolates were clustered together and little away from other published isolates of MRSA, Amino acid identities were 99.5% among the analyzed isolates, The three isolates shared 87.2%- 89.2% identity with other Staphylococcus aureus isolates
American Psychological Association (APA)
Hilmi, Salwa Muhammad& Nasif, Suad Abd al-Aziz Abd al-Wanis& Abu Rumaylah, Itab Muhammad& Ramadan, Ahmad Hijazi. 2015. Isolation and identification of methicillin resistant staphylococcus aureus (MRSA) from poultry meat and poultry products. Kafr El-Sheikh Veterinary Medical Journal،Vol. 13, no. 1, pp.57-69.
https://search.emarefa.net/detail/BIM-774156
Modern Language Association (MLA)
Hilmi, Salwa Muhammad…[et al.]. Isolation and identification of methicillin resistant staphylococcus aureus (MRSA) from poultry meat and poultry products. Kafr El-Sheikh Veterinary Medical Journal Vol. 13, no. 1 (2015), pp.57-69.
https://search.emarefa.net/detail/BIM-774156
American Medical Association (AMA)
Hilmi, Salwa Muhammad& Nasif, Suad Abd al-Aziz Abd al-Wanis& Abu Rumaylah, Itab Muhammad& Ramadan, Ahmad Hijazi. Isolation and identification of methicillin resistant staphylococcus aureus (MRSA) from poultry meat and poultry products. Kafr El-Sheikh Veterinary Medical Journal. 2015. Vol. 13, no. 1, pp.57-69.
https://search.emarefa.net/detail/BIM-774156
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 65-68
Record ID
BIM-774156