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Genotyping of staphylococcus aureus isolates based on methicillin-resistance genes and its relatedness to some putative virulence factors
Other Title(s)
التنميط الوراثي لعزلات بكتريا المكورات العنقودية الذهبية على أساس الموروثات المشفرة للمقاومة للمثيسلين و علاقتها ببعض عوامل الضراوة الشائعة
Joint Authors
Imad, Nadiyah
Abd al-Latif, Muhammad
Qandala, Nuha Juzif
Source
Issue
Vol. 58, Issue 2A (30 Jun. 2017), pp.626-638, 13 p.
Publisher
University of Baghdad College of Science
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) was a public health problem worldwide that causes nosocomial and community infections.
Forty three isolates (71.66%) were characterized as S.aureus, were isolated from 60 different clinical specimens (blood, nose, wound, urine and vaginal) collected from patients from different hospitals of Baghdad.
All isolates were resistant (100%) to Aztreonam, Carbenicillin, Cifixime, Cefoxitin, Ceftazidime, and showed high resistance to each of Methicillin, Oxacillin, Ampicillin and Penicillin .
the MRSA isolates were typed based on (SCCmec) typing ,the result revealed that SCCmecIVa was the most common in isolates (41.86%), following type IVc (20.93%), type II(16.27%).
Virulence factors were identified to detect genes encoding for Hlg,Pvl,ClfA,Tsst-1 and Eta, and the result showed the most prevalent gene was hlg (65.11%), following pvl (53.48%), clfA (51.16%), tsst-1(18.60%),eta (11.62%).
The virulence genes profiles were observed, and the most frequent was clfA-hlg-pvl (23.25%), clfA-hlg-pvl-tsst-1 (6.97%),clfA-hlg-tsst-1(4.65%).Analysis of genetic similarity relationship, showed the isolates of S.aureus were classified into two main clusters .
This result indicates that there a diversity in virulence genes profiles among MRSA isolates according to SCCmec types, and SCCmec IVa carried hlg, pvl , clfA genes was the most prevalent in Baghdad hospital isolates .
American Psychological Association (APA)
Qandala, Nuha Juzif& Abd al-Latif, Muhammad& Imad, Nadiyah. 2017. Genotyping of staphylococcus aureus isolates based on methicillin-resistance genes and its relatedness to some putative virulence factors. Iraqi Journal of Science،Vol. 58, no. 2A, pp.626-638.
https://search.emarefa.net/detail/BIM-775181
Modern Language Association (MLA)
Qandala, Nuha Juzif…[et al.]. Genotyping of staphylococcus aureus isolates based on methicillin-resistance genes and its relatedness to some putative virulence factors. Iraqi Journal of Science Vol. 58, no. 2A (2017), pp.626-638.
https://search.emarefa.net/detail/BIM-775181
American Medical Association (AMA)
Qandala, Nuha Juzif& Abd al-Latif, Muhammad& Imad, Nadiyah. Genotyping of staphylococcus aureus isolates based on methicillin-resistance genes and its relatedness to some putative virulence factors. Iraqi Journal of Science. 2017. Vol. 58, no. 2A, pp.626-638.
https://search.emarefa.net/detail/BIM-775181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 636-638
Record ID
BIM-775181