Survey and molecular detection of Melissococcus plutonius : the causative agent of European Foulbrood in honeybees in Saudi Arabia
Joint Authors
al-Waili, Nuri
Ayyad, Tahani H.
al-Ghamidi, Ahmad
Ansari, Muhammad Javid
Ahmad, Ashraf Muhammad
Nuru, Adgaba
al-Attal, Yahya Z.
al-Qurani, Abd al-Aziz Bin Sad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 6 (30 Sep. 2017), pp.1327-1335, 9 p.
Publisher
Publication Date
2017-09-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
A large-scale field survey was conducted to screen major Saudi Arabian beekeeping locations for infection by Melissococcus plutonius.
M.
plutonius is one of the major bacterial pathogens of honeybee broods and is the causative agent of European Foulbrood disease (EFB).
Larvae from samples suspected of infection were collected from different apiaries and homogenized in phosphate buffered saline (PBS).
Bacteria were isolated on MYPGP agar medium.
Two bacterial isolates, ksuMP7 and ksuMP9 (16S rRNA GenBank accession numbers, KX417565 and KX417566, respectively), were subjected to molecular identification using M.
plutonius -specific primers, a BLAST sequence analysis revealed that the two isolates were M.
plutonius with more than 98 % sequence identity.
The molecular detection of M.
plutonius from honeybee is the first recorded incidence of this pathogen in Saudi Arabia.
This study emphasizes the need for official authorities to take immediate steps toward treating and limiting the spread of this disease throughout the country.
American Psychological Association (APA)
Ansari, Muhammad Javid& al-Ghamidi, Ahmad& Nuru, Adgaba& Ahmad, Ashraf Muhammad& Ayyad, Tahani H.& al-Qurani, Abd al-Aziz Bin Sad…[et al.]. 2017. Survey and molecular detection of Melissococcus plutonius : the causative agent of European Foulbrood in honeybees in Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 24, no. 6, pp.1327-1335.
https://search.emarefa.net/detail/BIM-776415
Modern Language Association (MLA)
Ansari, Muhammad Javid…[et al.]. Survey and molecular detection of Melissococcus plutonius : the causative agent of European Foulbrood in honeybees in Saudi Arabia. Saudi Journal of Biological Sciences Vol. 24, no. 6 (Sep. 2017), pp.1327-1335.
https://search.emarefa.net/detail/BIM-776415
American Medical Association (AMA)
Ansari, Muhammad Javid& al-Ghamidi, Ahmad& Nuru, Adgaba& Ahmad, Ashraf Muhammad& Ayyad, Tahani H.& al-Qurani, Abd al-Aziz Bin Sad…[et al.]. Survey and molecular detection of Melissococcus plutonius : the causative agent of European Foulbrood in honeybees in Saudi Arabia. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 6, pp.1327-1335.
https://search.emarefa.net/detail/BIM-776415
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1334-1335
Record ID
BIM-776415