Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR
Joint Authors
Muhammad, Musa I.
Dawud, Turki M.
al-Mari, Khalid Salimin
Husayn, Ashgan M.
Jalal, Husayn M.
Kabli, Salih A.
Mubarak, Ayman Salim
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 2 (28 Feb. 2017), pp.367-370, 4 p.
Publisher
Publication Date
2017-02-28
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
The use of molecular techniques for detection and characterization of the Pasteurella multocida is very important for rapid and specific detection and characterization of the organism.
During the period from 15th February, 2014 to 15th April, 2015, 425 nasopharyngeal swabs and 175 lung and spleen samples were collected and examined by conventional methods, 80 strains (18.82%) of P.
multocida were isolated from the calves, sheep and goat with respiratory manifestation.
Meanwhile, 77 strains (44%) were isolated from emergency slaughtered animals.
All the recovered strains were positive for specific PCR for detection of P.
multocida strains previously identified as P.
multocida by standard microbiological techniques.
Multiplex PCR for molecular typing of the capsular antigens of the recovered P.
multocida revealed positive amplification of 1044 bp fragments specific to the capsular antigen type A with 105 strains (66.88%), and amplification 511 bp fragments of the capsular antigen type E with 52 strain (33.12%) and absence of B, D and F antigens
American Psychological Association (APA)
al-Mari, Khalid Salimin& Dawud, Turki M.& Mubarak, Ayman Salim& Husayn, Ashgan M.& Jalal, Husayn M.& Kabli, Salih A.…[et al.]. 2017. Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR. Saudi Journal of Biological Sciences،Vol. 24, no. 2, pp.367-370.
https://search.emarefa.net/detail/BIM-776455
Modern Language Association (MLA)
al-Mari, Khalid Salimin…[et al.]. Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR. Saudi Journal of Biological Sciences Vol. 24, no. 2 (Feb. 2017), pp.367-370.
https://search.emarefa.net/detail/BIM-776455
American Medical Association (AMA)
al-Mari, Khalid Salimin& Dawud, Turki M.& Mubarak, Ayman Salim& Husayn, Ashgan M.& Jalal, Husayn M.& Kabli, Salih A.…[et al.]. Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 2, pp.367-370.
https://search.emarefa.net/detail/BIM-776455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 370
Record ID
BIM-776455