The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action
Joint Authors
Kong, Shang
Ding, Chen
Huang, Lanlan
Bai, Yan
Xiao, Tiancun
Su, Zhengquan
Guo, Jiao
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 2 (28 Feb. 2017), pp.251-255, 5 p.
Publisher
Publication Date
2017-02-28
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
The objectives of this study were to explore the effect of COST (one thousand Da molecular weight chitosan oligosaccharide) on the differentiation of 3T3-L1 preadipocytes and to determine the mechanism of action.
3T3-L1 preadipocytes were used as the target cells, and the induction of the methods for the differentiation of 3T3-L1 preadipocytes was based on classic cocktails.
The MTT assay was used to filtrate the concentration of COST.
On the 6th day of induceddifferentiation, the differentiation of 3T3-L1 cells was detected by Oil Red O staining.
The expression of PPARc and C/EBPa mRNA was determined using real-time fluorescence quantitative PCR (Q-PCR).
COST inhibited 3T3-L1 preadipocyte differentiation in a dose-dependent manner and decreased lipid accumulation.
At the molecular level, the expression of the transcription factors, PPARc and C/EBPa, was reduced by COST during adipogenesis.
These results indicate that COST effectively inhibited the differentiation of 3T3-L1 preadipocytes.
The mechanism is related to the down-regulation expression of PPARc and C/EBPa
American Psychological Association (APA)
Kong, Shang& Ding, Chen& Huang, Lanlan& Bai, Yan& Xiao, Tiancun& Guo, Jiao…[et al.]. 2017. The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. Saudi Journal of Biological Sciences،Vol. 24, no. 2, pp.251-255.
https://search.emarefa.net/detail/BIM-776460
Modern Language Association (MLA)
Kong, Shang…[et al.]. The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. Saudi Journal of Biological Sciences Vol. 24, no. 2 (Feb. 2017), pp.251-255.
https://search.emarefa.net/detail/BIM-776460
American Medical Association (AMA)
Kong, Shang& Ding, Chen& Huang, Lanlan& Bai, Yan& Xiao, Tiancun& Guo, Jiao…[et al.]. The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 2, pp.251-255.
https://search.emarefa.net/detail/BIM-776460
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 255
Record ID
BIM-776460