Biochemical attributes of hens fed irradiated aflatoxin bi contaminated diet

Abstract EN

The purpose of this study is to evaluate the effect of feeding diet artificially contaminated with aflatoxin B1 (AFB1) at level 0.2 mg kg-1AFB1 ,and gamma(y) irradiated (10, 20, and 30 kGy)on reducing the deleterious effects of laying hens Golden Montaza (GM) biochemical attributes.

These include liver weight, AFB1 liver residue content, AST, ALT, ALP, creatinine, total proteins, albumin and globulin, as well as, the levels ofT3 , T4 , TSH, FSH, LH, progesterone hormone and hepatic histology.

At 38 week of age, groups of laying hens were fed on a normal non- contaminated diet (G1), aflatoxin-contaminated diet (G2 ), and irradiated contaminated diets (G3 , G4 and G5) for 3 weeks, as a duration period.

When the hens reached 42 weeks of age, they were fed on normal diet for 3 weeks, as a recovery period.

Results showed that AST, ALT, ALP, and creatinine significantly increased in AFs treated groups in comparison with those received AFs-containing diet and irradiated up to 30 kGy.

Layers fed contaminated diet of AFB1 suffered from a lower level of total proteins, albumin and globulin .Meanwhile, the results showed that the level of serum T4was lower, but conversely the levels of FSH were higher for those fed on diets contaminated with AFB1compared to those fed irradiated contaminated diets with AFB1, no significant change occurred in serum blood T3 , TSH, LH and progesterone in all tested groups.

Treated contaminated diets withyirradiation at 30 kGy reduced the incidence and severity of hepatic histology.

The 30 kGy radiation dose was more effective, in this respect, in all biochemical indices.

For recovery period diets non-contaminated withAFB1, the results showed improvements in all biochemical indices and recovered the hepatic structure with increasing the recovery period especially for those fed on irradiated diets through the experimental duration.

In conclusion, feeding of diets contaminated with AFB1 altered the blood profiles, and damaged the liver tissues in laying hens compared to irradiation treatment of diets, which was probably responsible for recovering the biochemical alterations in laying hens