Association between interleukin-4 (IL-4)‎, gene polymorphisms (C-589T, T+2979G, and C-33T)‎ and migraine susceptibility in Iranian population : a case-control study

Abstract EN

Background : Migraine is a chronic neurological disease characterized by recurrent moderate to severe headaches commonly in association with neuro-inflammation.

Interleukin-4 (IL-4), an anti-inflammatory cytokine, plays an important role in modulating pain threshold and has an essential role in stimulation of pain receptors in the trigeminal nerve fibers.

Aim of the study : The current study aimed to investigate the possible associations between IL-4 single nucleotide polymorphisms (SNPs) and susceptibility to migraine in Iranian patients.

Patients and methods: In a prospective case–control study, we studied blood samples of 190 patients with migraine (migraineurs) and 200 healthy controls (HCs) for analysis of gene variants.

Genotyping for the IL-4 SNPs: C-589T (rs2243250), T+2979G (rs2227284), and C-33T (rs2070874) were performed using PCR-RFLP.

Statistical analysis was performed using the SPSS version 21.0 (SPSS, Chicago) and SNPStats version 1.14.0.

Results and conclusion : Among IL-4 SNPs, rs2243250 (TC genotype, OR= 0.25, 95% CI= 0.13–0.50, P = 0.001) and rs2227284 (TG and TT genotypes, OR= 0.44, 95% CI= 0.23– 0.92, P = 0.029 and OR = 0.38, 95% CI= 0.18–0.79, P = 0.009 respectively) were significantly