Isolation and molecular characterization of foot and mouth disease virus in Kafrelsheikh Governorate, Egypt 2014
Other Title(s)
التوصيف الجزيئي و التتابع النيكلوتيدي لفيروس الحمى القلاعية في مصر 2014
Joint Authors
Qasim, Sami
Majuza, Asma
al-Baiuri, Jabr Fekryb
Zaghlul, Abir
Abd al-Ati, Majdi
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 13, Issue 2 (31 Dec. 2015), pp.17-35, 19 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Foot and mouth disease virus (FMDV) is the most economically important veterinary pathogen due to its highly infectious nature and ability to cause persistent infection.In the period betweenAugust to October2014, an outbreak ofFoot and Mouth Disease(FMD) occurred in Egypt.
This study was designated to isolate and detect the current strains of FMDV circulating in Egypt.
Twenty sixtissue samples were collected from clinically diseased cattle and buffaloes from Kafrelsheikh province.The isolation of the causative agent wasdone by inoculation of baby mice intraperitoneally then passed to Baby Hamster Kidney-21 (BHK-21) cells.
The inoculated mice showed paralysis and death, while the infected BHK-21 showed clear CPE (cell rounding and cell death).
Molecular characterizations of the FMDV were doneusing universal primers followed by serotype specific primers for A, O, C and SAT2.
Only serotypes SAT2 and A were detected with an overall detection rate of 53.8%.Results of phylogenetic tree of the current FMDV strains revealed that there is no significant divergence among the recent isolated strains and other Egyptian isolated strains from different localities
American Psychological Association (APA)
Qasim, Sami& Majuza, Asma& al-Baiuri, Jabr Fekryb& Abd al-Ati, Majdi& Zaghlul, Abir. 2015. Isolation and molecular characterization of foot and mouth disease virus in Kafrelsheikh Governorate, Egypt 2014. Kafr El-Sheikh Veterinary Medical Journal،Vol. 13, no. 2, pp.17-35.
https://search.emarefa.net/detail/BIM-777832
Modern Language Association (MLA)
Qasim, Sami…[et al.]. Isolation and molecular characterization of foot and mouth disease virus in Kafrelsheikh Governorate, Egypt 2014. Kafr El-Sheikh Veterinary Medical Journal Vol. 13, no. 2 (2015), pp.17-35.
https://search.emarefa.net/detail/BIM-777832
American Medical Association (AMA)
Qasim, Sami& Majuza, Asma& al-Baiuri, Jabr Fekryb& Abd al-Ati, Majdi& Zaghlul, Abir. Isolation and molecular characterization of foot and mouth disease virus in Kafrelsheikh Governorate, Egypt 2014. Kafr El-Sheikh Veterinary Medical Journal. 2015. Vol. 13, no. 2, pp.17-35.
https://search.emarefa.net/detail/BIM-777832
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 30-34
Record ID
BIM-777832