![](/images/graphics-bg.png)
Effect of bovine serum added to maturation medium on in vitro-maturation and some genes expressions of buffalo oocytes
Other Title(s)
تأثير إضافة السيرم البقري إلى بيئة البويضات الجاموسى معمليا على كل من الإنضاج و بعض التعبيرات الجينية المصاحبة
Joint Authors
Shamiyah, Sharif Muhammad
Fuad, Ayman Muhammad
Qadum, Abd al-Jawad Khalifah Abd al-Jawad
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 13, Issue 2 (31 Dec. 2015), pp.67-82, 16 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The effect of supplementing TCM-199 culture medium with bovine sera on buffalo’s oocytes maturation in vitro and expression of some related genes were studied.
A total 1149 immature buffalo oocytes were randomly divided into three groups, G1( n=315) were cultured in medium with no serum supplement(control), G2( n=531) were cultured in medium supplemented with 10% Fetal calf serum (FCS) and G3(n= 303) were cultured in medium supplemented with 10% Estrous cow serum (ECS).
After maturation, oocytes in each group were classified according to its shape and expansion into 2 subgroups, good (GO) and fair (FO) expanded oocytes.
Expression of GAPDH, CX43, IGF II and β- Actin genes were studied in each subgroup.
Results revealed that G3 and G2 showed high maturation rate (P<0.01 & 0.05 respectively) compared to G1(control) and ECS was superior (P<0.05) than FCS.
The qualitative expression of genes under study showed no difference among the studied groups, except CX43 gene expression was missed in FO in G1 concluding that addition of bovine sera especially estrus cow serum enhance in vitro maturation of the buffalo oocytes and CX4 gene expression and further studies is needed to enhance the expression of CX43 gene which might help in increasing maturation rate.
American Psychological Association (APA)
Shamiyah, Sharif Muhammad& Qadum, Abd al-Jawad Khalifah Abd al-Jawad& Fuad, Ayman Muhammad. 2015. Effect of bovine serum added to maturation medium on in vitro-maturation and some genes expressions of buffalo oocytes. Kafr El-Sheikh Veterinary Medical Journal،Vol. 13, no. 2, pp.67-82.
https://search.emarefa.net/detail/BIM-777835
Modern Language Association (MLA)
Shamiyah, Sharif Muhammad…[et al.]. Effect of bovine serum added to maturation medium on in vitro-maturation and some genes expressions of buffalo oocytes. Kafr El-Sheikh Veterinary Medical Journal Vol. 13, no. 2 (2015), pp.67-82.
https://search.emarefa.net/detail/BIM-777835
American Medical Association (AMA)
Shamiyah, Sharif Muhammad& Qadum, Abd al-Jawad Khalifah Abd al-Jawad& Fuad, Ayman Muhammad. Effect of bovine serum added to maturation medium on in vitro-maturation and some genes expressions of buffalo oocytes. Kafr El-Sheikh Veterinary Medical Journal. 2015. Vol. 13, no. 2, pp.67-82.
https://search.emarefa.net/detail/BIM-777835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 76-81
Record ID
BIM-777835