Protein analysis and heavy metals accumulation in muscles of wild and farmed Nile tilapia (Oreochromis Niloticus)‎

Abstract EN

The present study aimed to assess the levels of some heavy metals (Cu, Fe, Pb, Zn and Cd) in muscles of wild and farmed Oreochromisniloticusas well as to evaluate the human hazard index associated with fish consumption.

In addition, total protein,molecular weights and band counts of sarcoplasmic proteins were investigated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method.

The obtained results revealed that the accumulation of Cu has the highest value in farmed Oreochromisniloticus compared to wild Oreochromisniloticus while the highest Fe, Pb, Zn and Cd concentrations were recorded in wild Oreochromisniloticus compared to farmed Oreochromisniloticus.

The calculated hazard index (HI) indicated that all metals had low HI except Pb and Cd levels in both wild and farmedfish were higher than their permissible limits for fish as a human food.

There was no significant (P> 0.05) difference between wild and farmed Oreochromisniloticusin total protein.Wild fish predominant farmed fish in the number of separated proteins.

Wild fish muscle proteinshowed12 protein bands, whilefarmed fish muscle proteins showed 11 protein bands.

Also, these bands were differed in quantitative parameters.

Wild fish had unique bands (MW.

198.13, 97.92, 56.77 and 29.75) while farmed fish had unique bands (MW.

121.62, 79.05 and 26.16).

The current data foundthat there are differences in electrophoretic pattern and heavy metals accumulation between wild and farmed Oreochromisniloticus.