Molecular typing of clostridium perfringens isolates from soil, healthy, and diseased sheep in Egypt by multiplex PCR
Joint Authors
al-Sifi, A.
Akram, S.
Abidin, E.
Allam, M.
Nayil, M.
Hassan, H.
Source
Beni-Suef Veterinary Medical Journal
Issue
Vol. 22, Issue 1(s) (30 Jun. 2013), pp.53-57, 5 p.
Publisher
Beni-Suef University Faculty of Veterinary Medicine
Publication Date
2013-06-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
In this study multiplex PCR was used for typing of Clostridium perfringens isolates from soil, clinically healthy and diseased sheep.
Clostridium perfringens was isolated from 41 out of 100 soil samples, 12 out of 100 clinically healthy sheep and 118 out of 200 sheep with enterotoxaemia signs.
Genotyping of 41 isolates from soil indicated that 29 (70.73%) were type A, 3 (7.31%) were type B and 9 (21.95%) were type D.
Of 12 isolates from clinically healthy sheep 6 (50%) were type A and 6 (50%) were type D.
Of 118 isolates from diseased sheep 42 (35.59%) were type A, 22 (18.64%) were type B and 54 (45.76%) were type D.
This result indicates that Clostridium perfringens type A, B and D are the main types causing enterotoxaemia in sheep in Egypt and Clostridium perfringens
American Psychological Association (APA)
al-Sifi, A.& Akram, S.& Allam, M.& Hassan, H.& Abidin, E.& Nayil, M.. 2013. Molecular typing of clostridium perfringens isolates from soil, healthy, and diseased sheep in Egypt by multiplex PCR. Beni-Suef Veterinary Medical Journal،Vol. 22, no. 1(s), pp.53-57.
https://search.emarefa.net/detail/BIM-778692
Modern Language Association (MLA)
Nayil, M.…[et al.]. Molecular typing of clostridium perfringens isolates from soil, healthy, and diseased sheep in Egypt by multiplex PCR. Beni-Suef Veterinary Medical Journal Vol. 22, no. 1(s) (2013), pp.53-57.
https://search.emarefa.net/detail/BIM-778692
American Medical Association (AMA)
al-Sifi, A.& Akram, S.& Allam, M.& Hassan, H.& Abidin, E.& Nayil, M.. Molecular typing of clostridium perfringens isolates from soil, healthy, and diseased sheep in Egypt by multiplex PCR. Beni-Suef Veterinary Medical Journal. 2013. Vol. 22, no. 1(s), pp.53-57.
https://search.emarefa.net/detail/BIM-778692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 56-57
Record ID
BIM-778692