Molecular characterization of virulence genes in Escherichia coli isolated from newborn diarrheic calves

Joint Authors

Hisham, Nada S.
Shiashi, Talat H.
al-Shishini, Asma E.
al-Dasuqi, Ibrahim

Source

Kafr El-Sheikh Veterinary Medical Journal

Publisher

Kafr El-Sheikh University Faculty of Veterinary Medicine

Publication Date

2016-06-30

Country of Publication

Egypt

No. of Pages

22

Main Subjects

Veterinary Medicine

English Abstract

The present study was designed to elucidate the prevalence and characteristics of pathogenic Escherichia coli strains from diarrheic calves.

A total of 120 fecal samples were collected from diarrheic calves from different cow's farms at Kafrelsheikh Governorate, Egypt.

E.

coli was isolated with a percentage of 20% (24/120).

Serogrouping of E.coli isolates showed 12 types belong to different "O" serogroups; the most prevalent serogroups among isolates were O111, O103, O26, O55, O128, O15, O91 and O20.

A total of 12 E.

coli isolates were screened by multiplex PCR assay for the presence of virulence genes characteristic for E.coli, that is, shiga-toxin producing gene(s) (stx1, stx2), intimin (eaeA) and enterohemolysin (hlyA).

Out of the 12 isolates, 11 were positive for one or both stx1and stx2 (91.6%), 8 isolates for hlyA(66.6%) and 4 isolates for eaeA (33.3%).

PCR results showed that 9 (75%) isolates carried stx1 gene, 9 (75%) possessed stx2 gene while 7 isolates (58.3%) gave positive amplicon both for stx1 and stx2 genes.

All shiga toxin genes (stx1 and/or stx2) positive samples were positive for the hlyA and eaeA genes.

Both virulence genes eaeA and hlyA in the same sample were observed in 36.36% of the shiga toxin positive strains.

As a result, this study shows that stx1 and stx2 were found as the most common virulence gene markers of E.

coli strains isolated from calves with diarrhea

Data Type

Conference Papers

Record ID

BIM-778713

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