Phenotypic and genotyping characterization of Pasteurella Multocida in farm animals

Other Title(s)

التوصيف الظاهري و الوراثي للباستيريلا مالنوسيدا في حيوانات المزارع

Joint Authors

Ahmad, Ashraf Muhammad
Bustan, Tamir Ibrahim
Turki, Hilmi Ahmad
Hasan, Umniyah Fikri

Source

Kafr El-Sheikh Veterinary Medical Journal

Publisher

Kafr El-Sheikh University Faculty of Veterinary Medicine

Publication Date

2016-06-30

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Veterinary Medicine

English Abstract

Pasteurella multocida which is the common cause of respiratory disease in cattle causing high economic losses.

To achieve this objective, this study succeeded in isolating a total of 27 of P.

multocida isolates from 280 samples (9.6%).

Lung tissues showed the highest percentage of P.

multocida isolation (13.1%), followed by Nasal Swabs (5%).

These isolates were confirmed microscopically to be P.

multocida by staining with Gram's and Leishman's stains, then biochemically by traditional test like indole, catalase, oxidase, urease, citrate utilization, sugar fermentation and gelatin liquefaction tests.

Also, the results were confirmed using commercial test API 20E and PCR.

For common gene and capsular serotyping of P.

multocida by using multiplex capsular PCR typing system, a total of 9 representative isolates were tested and 5 isolates were positive for common gene of P.

multocida and positive for capsular type E.

Positive P.

multocida isolates were tested for presence of virulence factors by multiplex PCR for virulence genotyping which represented by virulence genes (TbpA, nanH, ompH and ptfA) which revealed 100% percentages of Tbpa and nanH in lung tissues and nasal swabs in cattle and buffaloes isolates, the presence of ptfA gene in high percentages in lung tissues in buffalo more than in nasal swabs.

Data Type

Conference Papers

Record ID

BIM-778714

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