Diagnosis of carrier state of foot and mouth disease virus in vaccinated and unvaccinated cattle by RT-PCR

Abstract EN

The aim of present work were to investigate the carrier state to foot and mouth disease virus in vaccinated cattle (vaccinated carriers) and unvaccinated cattle (unvaccinated carriers).

Reverse transcriptase polymerase chain reaction (RT-PCR) technique was employed to detect FMDV from esophageal and pharyngeal fluid samples collected from both vaccinated and unvaccinated cattle.

Results have been shown that the persistence of FMDV is significantly higher in unvaccinated cattle in comparison to vaccinated animals.

It have been concluded that high vaccination trials were play a role in the elimination of carrier status from cattle.