Pathogenetic effects of mercury chloride in white rats

Abstract EN

The study was performed on twelve white rats of approximately of the same body weight ( 200-220 gms) divided equally in to 3 groups ; The first group(T1) was received mercury chloride ( 1mg/kg B.W intraperitoneally once daily for 30 days).

While the second group (T2) was received mercury chloride (1.5mg/kg B.W intraperitoneally once daily for 30 days).Third group was received only0.2ml of Distilled water as control group.

At the end of experiment, the animals were sacrificed and small pieces of livers had been collected for genetic experiment.

Also small specimens (2cm³) were taken from livers and kidneys to histopathology.

The genetic experiment was showed that the T1 group demonstrated non-significant increase in p53 mRNA gene expression levels as compared with the control group, while the group T2 showed significant increase (p<0.05) in p53 mRNA gene expression level as compared with the control group.

The microscopic examination of histopathological sections of livers and kidneys of (T2) group was showed severe pathological changes characterized by vaculation and necrosis of hepatocyte and marked atrophy of glomeruli, degenerative changes of epithelial layer of renal tubules with cast formation, hemorrhage and congestion.

While (T1) group was showed less pathological changes in livers characterized by loss of radially arrangement of hepatocyte and dilatation of sinusoids.

In kidneys there was atrophy of glomeruli, hemorrhage and congestion.