Antimicrobial activity of local honey

Other Title(s)

النشاطية المضادة للبكتيريا في العسل و شمع العسل المحلي

Dissertant

al-Adayilah, Sundus Ahmad Mahmud

Thesis advisor

Khulayfat, Khalid Muhammad

Comitee Members

al-Shurafa, Khalid Yusuf Ahmad
al-Mustafa, Ahmad Husayn Husayn
al-Harub, Amir Munawwir

University

Mutah University

Faculty

Faculty of Science

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2017

English Abstract

In latest years an obvious raise in antibiotic resistance by inevitability pathogenic bacteria has been seen.

This rise is due to the overuse and misuse of antibiotics and has resulted in a number of highly resistant bacterial strain such as MRSA a strain of Staphylococcus aureus which only the antibiotic Vancomycin is currently effective.

In respect of contribution fighting bacterial infections, alternative procedures to traditional antibiotics should be explored.

This study was planned to discover the efficacy of honey and its propolis against Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus.cereus, Shigella, Salmonella typhimurium, Klebsiella aerogenes, Pseudomonas aeruginosa, Enterococcus faecalis and Escherichia coli.

In this study, the assessment of antibacterial activity was performed using two techniques agar-well diffusion method and broth macrodilution method.

The results showed major differences in the nature of the antibacterial activity when the two methods were compared.

For example, when using the agar-well diffusion method, the tried honey sample showed antibacterial activity only against Escherichia coli and Staphylococcus saprophyticus.

When using the broth macrodilution method, however, it was observed that this sample of honey showed inhibition of bacterial growth at lower concentrations and against other strains.

The bacteria tested showed the least amount of susceptibility to propolis with each producing a zone of inhibition less than 15mm.

S.

aureus showed 13mm zone of inhibition, B.

cereus 14mm.

The action of propolis was different from that of honey, through inhibiting the growth of Staphyllococcus aureus and Bacillus cereus, while its honey harvested from the same hives inhibited S.

saprophyticus and E.

coli.

Thus it is favorable to manage it as a mixture of both.

In DPPH system, the scavenging activities of local honey and propolis ranged from 9.81 to 90.7 % and from 25.33 to 73.47%, respectively, with IC50 values of 4.5 mg/ml and 7.5 μg/ml.

Because the S.

saprophyticus is the second-most common cause of community acquired UTIs, after Escherichia coli and during the intercourse exercise, that escalates the risk of S.

saprophyticus UTIs.

The locally available natural honey may be used as rich source of antioxidant and can be used as effective antibacterial agent in order to combat the bacterial infection to human particularly young females because of the UTI-causing infections for those ages are prevailing.

Main Subjects

Biology

No. of Pages

40

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-779164