Detection of Trichomonas vaginalis among women with abnormal vaginal discharge by PCR technique targeting TVK3 and TVK7 genes in Basrah province

Abstract EN

A total of 552 samples of vaginal discharge were collected from women that visited the Maternity and Pediatrics hospital in Basrah Province .Microscopic examination of vaginal discharge revealed that 4.1 % of these women were infected with Trichomoniasis .

In present diagnostic study, two technique were used to diagnosed the same sample for the first time in Iraq , these include microscopic examination ( wet preparation ) and Polymerase chain reaction (PCR) .PCR technique was the highest sensitivity ( 100 % ) which diagnosed three samples were considered negative in the other diagnostic technique and microscopic recorded sensitivity 88.4 % .In the same time diagnosis by vaginal discharge were found to be highly percent ( 100 % ) than the urine (1.9%) .