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The effectiveness of ELISA and PCR techniques for detecting cytomegalovirus DNA in blood donors
Joint Authors
Nanakaly, Haween T.
Husayn, Bashdar Mahmud
Qadir, Salim S.
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 29, Issue 1 (28 Feb. 2017), pp.1-5, 5 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2017-02-28
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Social Sciences (Multidisciplinary)
Abstract EN
Cytomegalovirus (CMV) is one of the most significant causes of transfusion- associated morbidity and mortality.
The infection with this virus may be asymptomatic and remain latent.
Serological screening of blood components may reduce the prevalence of transfusion- transmitted CMV (TT-CMV) infection, but still cases of TT-CMV infection in immuno- compromised patients are reported.
This study was designed to investigate the sensitivity of PCR technique compared to enzyme-linked immunosorbent assay (ELISA) for the detection of CMV in sera samples among blood donors in Erbil city.
Serum from 465 voluntary blood donors were screened for specific anti-CMV IgG and IgM by ELISA.
Fifty samples of them were selected to be tested more by PCR; 25 with positive and 25 negative for IgG anti-CMV antibodies.
Out of the 465 sera samples taken from blood donors, 148 (31.827%) were positive for IgG anti-CMV antibodies and negative for anti- CMV IgM antibodies.
CMV- DNA was detected in (92%) of anti CMV IgG positive, while DNA CMV in anti–CMV IgG negative samples was (64%) (p=o.ooo).
It is the time for PCR to substitute ELISA for screening of CMV in blood donors.
Detection of CMV in blood donors will reduce the spread of this infection which is a big burden on the health practice.
American Psychological Association (APA)
Nanakaly, Haween T.& Husayn, Bashdar Mahmud& Qadir, Salim S.. 2017. The effectiveness of ELISA and PCR techniques for detecting cytomegalovirus DNA in blood donors. ZANCO Journal of Pure and Applied Sciences،Vol. 29, no. 1, pp.1-5.
https://search.emarefa.net/detail/BIM-780007
Modern Language Association (MLA)
Husayn, Bashdar Mahmud…[et al.]. The effectiveness of ELISA and PCR techniques for detecting cytomegalovirus DNA in blood donors. ZANCO Journal of Pure and Applied Sciences Vol. 29, no. 1 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-780007
American Medical Association (AMA)
Nanakaly, Haween T.& Husayn, Bashdar Mahmud& Qadir, Salim S.. The effectiveness of ELISA and PCR techniques for detecting cytomegalovirus DNA in blood donors. ZANCO Journal of Pure and Applied Sciences. 2017. Vol. 29, no. 1, pp.1-5.
https://search.emarefa.net/detail/BIM-780007
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 4-5
Record ID
BIM-780007