Detection of listeria monocytogenes hlya gene in meat samples by real-time PCR in Erbil city

Abstract EN

The objectives of this study was to evaluate the detection of Listeria monocytogenes in different ready to eat foods using real-time PCR (RT-PCR).

To monitor L.

monocytogenes in meat Listeria monocytogenes is a gram positive, rod shaped, pathogenic bacterium 32 samples (imported frozen breast chicken,(17) local minced beef meat 9 and imported minced beef meat 6.

were collected from super markets in different place in Erbil city .

out of 32samples, 9 samples (28.1%) showed positive signals in RT-PCR.

six samples from(imported frozen breast chicken, (35.29%) ,1 samples from local minced beef meat(11.1%) and two sample from imported minced beef meat (33.3%) were determined to be positive for L.

monocytogenes .

Also the result showed the lowest CT value at (21.57) The results indicate that the RT-PCR detection method with the set of primers and hybridization probe designed in this study should be useful in monitoring for L.

monocytogenes hlyA gene in meat