Molecular characterization of glucosinolates and carotenoid biosynthetic genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Joint Authors
Arasu, Mariadhas Valan
Kim, Sun Ju
Chun, Jin Hyuk
Seo, Mi Suk
Jin, Mina
al-Dhabi, Nayif Abd Allah
Kim, Na Hyung
Park, Sang Un
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 1 (31 Jan. 2018), pp.71-82, 12 p.
Publisher
Publication Date
2018-01-31
Country of Publication
Saudi Arabia
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The present study aimed to investigate the contents of glucosinolates (GSLs) and carotenoids in eleven varieties of Chinese cabbage in relation to the expression level of the important transcription factors.
MS and HPLC analysis identified the presence of 13 GSLs (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, glucocochlearin, glucobrassicanapin, glucoerucin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and gluconasturtiin) and four carotenoids (lutein, zeaxanthin, a-carotene and b-carotene).
GSL contents were varied among the different cabbage varieties.
The total GSL content ranged from 2.7 to 57.88 lmol/g DW.
The proportion of gluconapin (54%) and glucobrassicanapin (22%) was higher in all the varieties, respectively.
Results documented the variation in total and individual carotenoid contents that have also been observed among different varieties; however, the total carotenoid contents ranged from 289.12 to 1001.41 mg kg 1 DW (mean 467.66).
Interestingly, the proportion of lutein (66.5) and b-carotene (25.9) were higher than a-carotene (5.1) and zeaxanthin (2.5 %).
Consequently, the expression level of the regulatory gene, MYB28 was higher in ‘K0648’ and was directly proportional to GSL content.
Similarly, the expression levels of 1-PSY were higher in ‘K0112’; however, the expression levels of 2-ZDS, 3-LCYB, 4-LCYE, 5-CHXB and 7-NCED genes showed no significant difference.
In addition, the correlation between GSL and carotenoid contents and gene expression level showed moderate significant difference in each Chinese cabbage.
American Psychological Association (APA)
Chun, Jin Hyuk& Kim, Na Hyung& Park, Sang Un& Arasu, Mariadhas Valan& Kim, Sun Ju& al-Dhabi, Nayif Abd Allah…[et al.]. 2018. Molecular characterization of glucosinolates and carotenoid biosynthetic genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Saudi Journal of Biological Sciences،Vol. 25, no. 1, pp.71-82.
https://search.emarefa.net/detail/BIM-780400
Modern Language Association (MLA)
al-Dhabi, Nayif Abd Allah…[et al.]. Molecular characterization of glucosinolates and carotenoid biosynthetic genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Saudi Journal of Biological Sciences Vol. 25, no. 1 (Jan. 2018), pp.71-82.
https://search.emarefa.net/detail/BIM-780400
American Medical Association (AMA)
Chun, Jin Hyuk& Kim, Na Hyung& Park, Sang Un& Arasu, Mariadhas Valan& Kim, Sun Ju& al-Dhabi, Nayif Abd Allah…[et al.]. Molecular characterization of glucosinolates and carotenoid biosynthetic genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 1, pp.71-82.
https://search.emarefa.net/detail/BIM-780400
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 81-82
Record ID
BIM-780400