Triterpenoid modulates the salt tolerance of lanosterol synthase deficient Saccharomyces cerevisiae, GIL77
Joint Authors
Inafuku, Masashi
Oku, Hirosuke
Basyuni, Muhammad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 1 (31 Jan. 2018), pp.1-9, 9 p.
Publisher
Publication Date
2018-01-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
This study examined the effect of triterpenoid on the salt tolerance of lanosterol synthase deficient yeast mutant GIL77.
The expression of the triterpenoid synthase gene under GAL1 promoter in GIL77 increased the triterpenoid concentration of both whole cell and plasma membrane fractions.
Without the induction of the genes, the growth curve of BgbAS or RsM1 transformant depicted patterns similar to control cells in both the presence and absence of salt with growth inhibition at 500 mM NaCl.
The induction of BgbAS and RsM1 gene expression slightly repressed growth compared with control cells in the absence of NaCl.
The growth of GIL77 was significantly suppressed by the expression of BgbAS or RsM1 under salinity conditions.
Of the triterpenoid synthase genes, BgbAS rather than RsM1 was found to strongly inhibit the growth of GIL77 cells under salt stressed conditions.
The expression of the triterpenoid synthase gene in GIL77 also influenced their tolerance to other abiotic stresses.
In contrast to the endogenous synthesis, the exogenous supply of triterpenoid in the culture medium appeared to occur in the plasma membrane fraction and enhanced the salt tolerance of GIL77.
This study thus discussed the physiological significance of triterpenoid in relation to its possible role in modulating salt tolerance.
American Psychological Association (APA)
Inafuku, Masashi& Basyuni, Muhammad& Oku, Hirosuke. 2018. Triterpenoid modulates the salt tolerance of lanosterol synthase deficient Saccharomyces cerevisiae, GIL77. Saudi Journal of Biological Sciences،Vol. 25, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-780413
Modern Language Association (MLA)
Inafuku, Masashi…[et al.]. Triterpenoid modulates the salt tolerance of lanosterol synthase deficient Saccharomyces cerevisiae, GIL77. Saudi Journal of Biological Sciences Vol. 25, no. 1 (Jan. 2018), pp.1-9.
https://search.emarefa.net/detail/BIM-780413
American Medical Association (AMA)
Inafuku, Masashi& Basyuni, Muhammad& Oku, Hirosuke. Triterpenoid modulates the salt tolerance of lanosterol synthase deficient Saccharomyces cerevisiae, GIL77. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-780413
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-9
Record ID
BIM-780413