5, 7-Dimethoxycoumarin prevents chronic mild stress induced depression in rats through increase in the expression of heat shock protein-70 and inhibition of monoamine oxidase-A levels

Abstract EN

The current study was aimed to investigate the role of 5,7-dimethoxycoumarin in the prevention of chronic mild stress induced depression in rats.

The chronic mild stress rat model was prepared using the known protocols.

The results from open-field test showed that rats in the chronic mild stress group scored very low in terms of crossings and rearings than those of the normal rats.

However, pre-treatment of the rats with 10 mg / kg doses of 5,7-dimethoxycoumarin prevented decline in the locomotor activity by chronic mild stress.

The level of monoamine oxidase-A in the chronic mild stress rat hippocampus was markedly higher.

Chronic mild stress induced increase in the monoamine oxidase-A level was inhibited by pre-treatment with 10 mg/kg doses of 5,7- dimethoxycoumarin in the rats.

Chronic mild stress caused a marked increase in the level of caspase-3 mRNA and proteins in rat hippocampus tissues.

The increased level of caspase-3 mRNA and protein level was inhibited by treatment of rats with 5,7- dimethoxycoumarin (10 mg / kg).

5,7-Dimethoxycoumarin administration into the rats caused a marked increase in the levels of heat shock protein-70 mRNA and protein.

The levels of heat shock protein-70 were markedly lower both in normal and chronic mild stress groups of rats compared to the 5,7-dimethoxycoumarin treated groups.

Thus 5,7-dimethoxycoumarin prevented the chronic mild stress induced depression in rats through an increase in the expression of heat shock protein-70 and inhibition of monoamine oxidase-A levels.