Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia
Joint Authors
al-Ansi, Salih
Tarroum, Muhammad
al-Amiri, Abd al-Hafiz A.
Abd al-Rahman, Jafar Z.
al-Shammari, Arif
Nadim, Muhammad
Khan, Salim
al-Quraini, Fahd
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 1 (31 Jan. 2018), pp.111-116, 6 p.
Publisher
Publication Date
2018-01-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia.
Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings.
Therefore, alternative method of tissue culture was attempted to regenerate and multiply the plant.
High shoot regeneration (14.44 shoots/explant) was obtained after four weeks, when shoot cuttings cultured on MS containing BA at 1.0 mM.
Other cytokinins e.g., Kn, 2iP and TDZ found to be less effective in bud induction and shoot multiplication.
Individual shoots were rooted on MS medium supplemented with various auxins at 0.5–5.0 mM concentrations.
The IBA (1.5 mM) supplemented MS media induced maximum (83.3 %) rooting.
The plantlets were acclimatized and hardened under greenhouse conditions in plastic pots containing soil and farm yard manure with 95.0 % success.
The protocol developed would help to multiply the plant as well as conserve them in natural habitat.
This can also be utilized to obtain active constituents for pharmaceutics and genetic manipulations.
American Psychological Association (APA)
Nadim, Muhammad& Khan, Salim& al-Ansi, Salih& Tarroum, Muhammad& al-Amiri, Abd al-Hafiz A.& Abd al-Rahman, Jafar Z.…[et al.]. 2018. Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 25, no. 1, pp.111-116.
https://search.emarefa.net/detail/BIM-780522
Modern Language Association (MLA)
Nadim, Muhammad…[et al.]. Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi Journal of Biological Sciences Vol. 25, no. 1 (Jan. 2018), pp.111-116.
https://search.emarefa.net/detail/BIM-780522
American Medical Association (AMA)
Nadim, Muhammad& Khan, Salim& al-Ansi, Salih& Tarroum, Muhammad& al-Amiri, Abd al-Hafiz A.& Abd al-Rahman, Jafar Z.…[et al.]. Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 1, pp.111-116.
https://search.emarefa.net/detail/BIM-780522
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 115-116
Record ID
BIM-780522