Effects of Decitabine on the proliferation of K562 cells and the expression of DR4 gene
Joint Authors
Zhang, Wenhui
Pei, Xiaohang
Zang, Yuzhu
Han, Shuangyin
Chen, Yuqing
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 2 (28 Feb. 2018), pp.242-247, 6 p.
Publisher
Publication Date
2018-02-28
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Objective : To investigate the role of DR4 gene in the occurrence, development and prognosis of acute myeloid leukemia (AML), find a new regulatory gene of Decitabine for the treatment of AML, namely DR4 gene, and explore the molecular mechanism of AML in the treatment of AML.
Methods : The methylation level and the mRNA expression level of DR4 gene promoters of bone marrow mononuclear cells in 122 patients with newly diagnosed AML and 24 patients with iron deficiency anemia (IDA) were detected using Methylation specific PCR (MS-PCR) and Q-RT-PCR, respectively, and a correlation analysis of them was conducted.
The effects of Decitabine on the proliferation of K562 cells were detected using CCK-8 assay.
Then, the effects of Decitabine on the methylation level and the Mrna expression level of DR4 genes of K562 cells treated with Decitabine were detected using MS-PCR and Q-RT-PCR, respectively.
The effects of Decitabine on the cell cycle and apoptosis of K562 cells were detected using flow cytometry.
Results : Compared with the control group, the methylation level (P = .002) of DR4 genes of bone marrow mononuclear cells in patients with newly diagnosed AML was high.
The methylation level (P = .01) of DR4 genes of bone marrow mononuclear cells in patients of the positive group of enlargement of liver, spleen and lymph node was lower than that of the negative group, and the methylation level (P = .006) of DR4 genes in patients of the high risk group of clinical stage was lower than that of the low risk group, and the methylation level (P = .03) of DR4 genes in patients of the group where patients did not achieve complete remission (CR1) after a course of induction chemotherapy was lower than that of the group where patients achieved complete remission (CR1) after a course of induction chemotherapy.
There was a significant negative correlation (P < .01) between the methylation level and the mRNA expression level of DR4 genes of bone marrow mononuclear cells in 122 patients with newly diagnosed AML.
After the K562 cells were treated with Decitabine for 48 h, the methylation level of DR4 gene promoters gradually decreased, while the mRNA expression level of DR4 genes gradually increased, both of which showed a concentration-dependent relationship.
After the K562 cells were treated with 5 mmol / L Decitabine for 48 h, the K562 cells in G0 / G1 phase and G2 / M phase increased significantly, and the K562 cells in S phase decreased significantly.
Conclusion : DR4 gene played an important role in the occurrence and development of AML.
Decitabine can effectively inhibit the proliferation of K562 cells, which probably partly because it can terminate the methylation effect of DR4 gene promoters and restore the mRNA expression of DR4 genes.
American Psychological Association (APA)
Zhang, Wenhui& Chen, Yuqing& Pei, Xiaohang& Zang, Yuzhu& Han, Shuangyin. 2018. Effects of Decitabine on the proliferation of K562 cells and the expression of DR4 gene. Saudi Journal of Biological Sciences،Vol. 25, no. 2, pp.242-247.
https://search.emarefa.net/detail/BIM-780585
Modern Language Association (MLA)
Zhang, Wenhui…[et al.]. Effects of Decitabine on the proliferation of K562 cells and the expression of DR4 gene. Saudi Journal of Biological Sciences Vol. 25, no. 2 (Feb. 2018), pp.242-247.
https://search.emarefa.net/detail/BIM-780585
American Medical Association (AMA)
Zhang, Wenhui& Chen, Yuqing& Pei, Xiaohang& Zang, Yuzhu& Han, Shuangyin. Effects of Decitabine on the proliferation of K562 cells and the expression of DR4 gene. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 2, pp.242-247.
https://search.emarefa.net/detail/BIM-780585
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 246-247
Record ID
BIM-780585