Overexpression of BAS1 in rice blast fungus can promote blast fungus growth, sporulation and virulence in planta
Joint Authors
Wang, Chunmei
Yang, Jing
Wang, Yunfen
Yan, Jinlu
Wang, Changmi
Li, Chengyun
Liu, Yanqin
Liu, Lin
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 8 (31 Dec. 2017), pp.1884-1893, 10 p.
Publisher
Publication Date
2017-12-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Background : BAS1 is biotrophy-associated secreted protein of rice blast strain (Magnaporthe oryzae).
In order to study the effect of BAS1 on virulence of rice blast strain, we characterized function of BAS1 using a purified prokaryotic expression product of BAS1 and its overexpression strain.
Results: Our results showed in vitro the purified expression product caused rapid callose deposition and ROS production in rice leaves and calli, indicated it triggered transient basal defense.
When the purified expression product of BAS1 was sprayed onto rice leaves, and 24 h later the leaves were inoculated with blast strain, the results showed the size and number of lesions, on purified BAS1 product-pretreated leaves of the Lijiangxintuanheigu (LTH) challenged with blast strain, was higher than those in BAS1-untreated leaves directly challenged with the same strain, which suggested the defense response trigged by BAS1 can be overcome by other effectors of the fungus.
More severe symptoms, higher sporulation, higher relative fungal growth and more lower expression level of defense-related genes appeared in LTH leaves challenged with overexpression strain 35S : BAS1 / Mo-2 than those in LTH inoculated with wild-type strain.
Conclusions : these data suggest both in vitro pretreatment with BAS1 prokaryotic expression products and overexpression in blast strains can increase virulence of blast fungus.
American Psychological Association (APA)
Yang, Jing& Liu, Lin& Wang, Yunfen& Wang, Chunmei& Yan, Jinlu& Wang, Changmi…[et al.]. 2017. Overexpression of BAS1 in rice blast fungus can promote blast fungus growth, sporulation and virulence in planta. Saudi Journal of Biological Sciences،Vol. 24, no. 8, pp.1884-1893.
https://search.emarefa.net/detail/BIM-780616
Modern Language Association (MLA)
Yang, Jing…[et al.]. Overexpression of BAS1 in rice blast fungus can promote blast fungus growth, sporulation and virulence in planta. Saudi Journal of Biological Sciences Vol. 24, no. 8 (Dec. 2017), pp.1884-1893.
https://search.emarefa.net/detail/BIM-780616
American Medical Association (AMA)
Yang, Jing& Liu, Lin& Wang, Yunfen& Wang, Chunmei& Yan, Jinlu& Wang, Changmi…[et al.]. Overexpression of BAS1 in rice blast fungus can promote blast fungus growth, sporulation and virulence in planta. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 8, pp.1884-1893.
https://search.emarefa.net/detail/BIM-780616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1892-1893
Record ID
BIM-780616