Function of tumor necrosis factor alpha before and after mutation in gastric cancer

Abstract EN

Purpose : to explore the cause of functional changes of tumor necrosis factor alpha (TNF-a) in development of gastric cancer through the structural changes of each site in TNF-a before and after mutation.

Methods : three typical mutant sites (TNF-a-308G / A, 857C / T and 863C / A of TNF-a) were chosen and methods like ab initio modeling was adopted for 3D modeling of TNF-a before and after mutation, besides, the structural changes were also analyzed.

Results: Mutation of TNF-a-308G / A led to the production of multiple helical structures and that of 863C / A caused the production of one helical structure in its adjacent region.

Mutation of 857C / T, however, did not cause the change in the basic structure of TNF-a.

Conclusions : Structural changes of TNF-a may have a significant effect on development of gastric cancer.