Epidemiological and molecular studies on infectious bursal disease virus infection in Luxor province
Other Title(s)
دراسات وبائية و جزيئية على مرض التهاب فبريس المعدي في محافظة الأقصر
Joint Authors
Uthman, Nabilah
Ibrahim, Inas Muhammad
Ibrahim, Ahmad I.
Nasif, Suad Abd al-Aziz Abd al-Wanis
Source
Assiut Veterinary Medical Journal
Issue
Vol. 63, Issue 153 (30 Apr. 2017), pp.54-59, 6 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The infectious bursal disease (IBD) is a continuing serious problem facing poultry industry in Egypt.
In this study, 500 bursae samples were collected from different broiler flocks in different localities of Luxor Governorate.
The flocks were suffering from mortality and bursal lesions during Dec.2014 to Jan.
2016.
The samples were tested by AGPT and the result was 12 flocks only were positive for IBD and then the positive samples were isolated in SPF embryonated chicken eggs.
All the inoculated embryos died within 2-3 days.
The embryos were smaller than normal, congested with haemorrhagic head, and necrotic foci of liver.
Then the presence of virus in embryonated eggs was confirmed by convential PCR.
Furthermore, molecular characterization was performed by direct sequencing of a 620-bp cDNA corresponding to the VP2 variable domain of the polyprotein gene synthesized by PCR.
With deduced amino acid analysis found that all examined isolates are very virulent strains.
four local strains used for nucleotide sequence, percent identity and phylogenetic tree analysis revealed that four isolates (F21,F23,F24,F26) were very close to very virulent old Egyptian strains Giza 2008.
American Psychological Association (APA)
Ibrahim, Inas Muhammad& Uthman, Nabilah& Ibrahim, Ahmad I.& Nasif, Suad Abd al-Aziz Abd al-Wanis. 2017. Epidemiological and molecular studies on infectious bursal disease virus infection in Luxor province. Assiut Veterinary Medical Journal،Vol. 63, no. 153, pp.54-59.
https://search.emarefa.net/detail/BIM-780746
Modern Language Association (MLA)
Ibrahim, Inas Muhammad…[et al.]. Epidemiological and molecular studies on infectious bursal disease virus infection in Luxor province. Assiut Veterinary Medical Journal Vol. 63, no. 153 (Apr. 2017), pp.54-59.
https://search.emarefa.net/detail/BIM-780746
American Medical Association (AMA)
Ibrahim, Inas Muhammad& Uthman, Nabilah& Ibrahim, Ahmad I.& Nasif, Suad Abd al-Aziz Abd al-Wanis. Epidemiological and molecular studies on infectious bursal disease virus infection in Luxor province. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 153, pp.54-59.
https://search.emarefa.net/detail/BIM-780746
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 58-59
Record ID
BIM-780746