An outbreek of aflatoxicosis in clarias gariepinus : a mycological, biochemical and histopathological studies

Other Title(s)

انتشار التسمم بالأفلاتوكسين في أسماك القرموط المستزرعة : دراسات فطرية و بيوكيميائية و باثولوجية

Joint Authors

Khidr, Zaghlul Ahmad Abd al-Munim
al-Matari, Dua Ahmad Husayn
Adawi, Rawiyah Sad Muhammad
Dib, Khalid Abd al-Rahman al-Gharib

Source

Assiut Veterinary Medical Journal

Issue

Vol. 63, Issue 152 (31 Jan. 2017), pp.135-145, 11 p.

Publisher

Assiut University Faculty of Veterinary Medicine

Publication Date

2017-01-31

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Natural & Life Sciences (Multidisciplinary)

Abstract EN

A total of one hundred and twenty cat fish (Clarias gariepinus) were collected from two private fish farms in Dakahlia governorate during summer, 2016.

We collected fifty diseased fish from first farm which suffered from cumulative mortality and seventy healthy fish from second farm.

All collected fish were subjected to clinical, postmortem, histopathological and mycological examination.

Aflatoxins were extracted and quantitated from feed and musculature of diseased and healthy fish (5 samples for each) using HPLC.

Blood samples were collected for biochemical and hematological parameters.

The clinical observation revealed that the diseased fish showed opacity of the eyes, paleness of the gills with presence yellowish greenish infiltration, hemorrhagic, yellowish skin and fins, and the fish were slow in motion.

The postmortem examination showed enlargement of the gall bladder, distention of the stomach, yellowish enlarged liver, swollen, dark red and friable kidneys.

Whereas the histopathological examination revealed a severe degenerative and necrotic changes in most internal organs of diseased fish.

On the other hand mycological examination of diseased fish yielded isolation of Aspergillusflavus (42%), A.

parasiticus (34.8%), A.

niger (12%) and A.

fumigatus (10.9%).

While no fungi was isolated from any organs of healthy fish.

The mean levels of aflatoxins in feed of diseased fish was 108.9±0.77 ppb (46.5±0.75 ppb AFB1, 1.4±0.11 ppb AFB2, 58.9±0.38 ppb AFG1, 2.1±0.24 ppb AFG2), while the mean levels of AFs residues detected in musculature of diseased fish was 20.9±0.39 ppb (11.3±0.25 AFB1, 0.15±0.007 AFB2, 8.9±0.20 AFG1, 0.5±0.44 AFG2).

Whereas Aflatoxins not detectable in feed or muscles of healthy fish.

Regarding to biochemical and hematological parameters, there were significant decrease in serum protein and albumin also a significant increase in AST, ALT and serum creatinine, as well as there were significant decrease in HB concentration and RBCs count of diseased fish compared to healthy fish.

American Psychological Association (APA)

al-Matari, Dua Ahmad Husayn& Adawi, Rawiyah Sad Muhammad& Khidr, Zaghlul Ahmad Abd al-Munim& Dib, Khalid Abd al-Rahman al-Gharib. 2017. An outbreek of aflatoxicosis in clarias gariepinus : a mycological, biochemical and histopathological studies. Assiut Veterinary Medical Journal،Vol. 63, no. 152, pp.135-145.
https://search.emarefa.net/detail/BIM-781322

Modern Language Association (MLA)

al-Matari, Dua Ahmad Husayn…[et al.]. An outbreek of aflatoxicosis in clarias gariepinus : a mycological, biochemical and histopathological studies. Assiut Veterinary Medical Journal Vol. 63, no. 152 (Jan. 2017), pp.135-145.
https://search.emarefa.net/detail/BIM-781322

American Medical Association (AMA)

al-Matari, Dua Ahmad Husayn& Adawi, Rawiyah Sad Muhammad& Khidr, Zaghlul Ahmad Abd al-Munim& Dib, Khalid Abd al-Rahman al-Gharib. An outbreek of aflatoxicosis in clarias gariepinus : a mycological, biochemical and histopathological studies. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 152, pp.135-145.
https://search.emarefa.net/detail/BIM-781322

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 142-145

Record ID

BIM-781322