Hematological and biochemical improvement by catechin and edta in lead intoxicated rats
Other Title(s)
تحسين التغيرات الدموية و البيوكيميائيه بواسطة الكاتشين و الأديتا في الفئران المتسممة بالرصاص
Joint Authors
Sharqawi, Ahmad Abd al-Baqi
Umar, Husam al-Din Muhammad
Muhammad, Wafa Hasanayn
Sayyid, Manal Muhammad
Ahmad, Duha Yahya
Source
Assiut Veterinary Medical Journal
Issue
Vol. 63, Issue 152 (31 Jan. 2017), pp.161-172, 12 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2017-01-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The present study was conducted to evaluate the efficacy of both Catechin and Calcium Disodium Ethylene diamine Tetraacetic Acid (CaNa2EDTA) in treatment of long-term lead toxicity through assessment of some biochemical indices (hematological picture and biochemical parameters).
Eighty male albino rats weighting 100-150 g of 10-12 weeks old were randomly divided into 4 groups (20 each).
Group 1 was left without any treatment as negative control group.
The other three groups (G2, G3 and G4) were exposed to lead acetate in drinking water at a concentration of 30 mg/L for 3 months.
G2 was used as positive conrol group.
G3 was divided into three subgroups A, B & C was treated with catechin in drinking water at a concentration of 49 mg/L for 7 days after 1, 2 and 3 month after Pb exposure, respectively.
G4 was also divided into three subgroups ( D, E & F ) and treated through IP injection CaNa2EDTA in a dose of 50 mg/kg body weight, for 5 days after 1, 2 & 3 months of lead exposure.
Six rats were taken randomly after 30, 60 and 90 days from negative and positive controls, 37, 67, 97 days from rats treated with catechin and 35, 65 and 95 days from rats treated with CaNa2EDTA.
Rats were anesthetized with ether and sacrified for blood and tissues collection.
Blood samples were collected in vacutainer tubes containing EDTA as anticoagulant for hematological pictures.
Brain tissue samples were collected from each rat for the subsequent biochemical parameters (total protein, nitric oxide, lipid peroxidation, glutathione, superoxide dismutase, catalase, glucose 6 phosphatase dehydratase and, acetylcholinestrase activity).
The results revealed that administration of catechin or CaNa2EDTA can minimize any toxic effects on hematological picture & biochemical parameters.
Catechin was more effective than CaNa2EDTA in improvement of Hb concentration, HCT %, MCV, MCH values and NO, LPO parameters but CaNa2EDTA was more effective than Catechin in improvement of WBCs, lymphocyte, monocyte count and GSH, SOD, CAT activities.
American Psychological Association (APA)
Muhammad, Wafa Hasanayn& Sayyid, Manal Muhammad& Sharqawi, Ahmad Abd al-Baqi& Ahmad, Duha Yahya& Umar, Husam al-Din Muhammad. 2017. Hematological and biochemical improvement by catechin and edta in lead intoxicated rats. Assiut Veterinary Medical Journal،Vol. 63, no. 152, pp.161-172.
https://search.emarefa.net/detail/BIM-781577
Modern Language Association (MLA)
Muhammad, Wafa Hasanayn…[et al.]. Hematological and biochemical improvement by catechin and edta in lead intoxicated rats. Assiut Veterinary Medical Journal Vol. 63, no. 152 (Jan. 2017), pp.161-172.
https://search.emarefa.net/detail/BIM-781577
American Medical Association (AMA)
Muhammad, Wafa Hasanayn& Sayyid, Manal Muhammad& Sharqawi, Ahmad Abd al-Baqi& Ahmad, Duha Yahya& Umar, Husam al-Din Muhammad. Hematological and biochemical improvement by catechin and edta in lead intoxicated rats. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 152, pp.161-172.
https://search.emarefa.net/detail/BIM-781577
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 170-172
Record ID
BIM-781577