Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene
Joint Authors
Ayyad, Tahani H.
al-Dakhil, Abir A.
al-Ajami, Rim A.
Siddiqi, Nikhat J.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 7 (30 Nov. 2017), pp.1697-1703, 7 p.
Publisher
Publication Date
2017-11-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Studies on the distribution of sand flies are important for the control of leishmaniasis in endemic and neighboring areas.
In the present study polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) was used to identify the distribution of sand flies in Al- Madinah and Asir Regions of Saudi Arabia using PCR–RFLP of 18S ribosomal RNA gene.
Based on the morphological characteristics, the sand flies were differentiated into seven species viz., Phlebotomus papatasi, Phlebotomus sergenti, Phlebotomus bergeroti, Sergentomyia clydei, Sergentomyia antennata, Sergentomyia fallax and Sergentomyia schwetzi.
PCR–RFLP of 18S ribosomal RNA (rRNA) genes with eight different restriction enzymes resulted in species-specific agarose gel electrophoresis banding patterns.
Of the eight restriction enzymes used, not a single restriction enzyme by itself could separate species belonging to the same genera (like P.
papatasi and P.
sergenti by AseI) as well as those belonging to different genera (like P.
papatasi and S.
clydei by AseI).
We therefore conclude that the genetic diversity within sand fly species based on PCR–RFLP technique was nonspecific.
Studies are in progress to study the viability of alternate techniques like lowstringency single specific primer polymerase chain reaction which can be used for molecular typing.
American Psychological Association (APA)
al-Dakhil, Abir A.& al-Ajami, Rim A.& Siddiqi, Nikhat J.& Ayyad, Tahani H.. 2017. Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene. Saudi Journal of Biological Sciences،Vol. 24, no. 7, pp.1697-1703.
https://search.emarefa.net/detail/BIM-781636
Modern Language Association (MLA)
al-Dakhil, Abir A.…[et al.]. Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene. Saudi Journal of Biological Sciences Vol. 24, no. 7 (Nov. 2017), pp.1697-1703.
https://search.emarefa.net/detail/BIM-781636
American Medical Association (AMA)
al-Dakhil, Abir A.& al-Ajami, Rim A.& Siddiqi, Nikhat J.& Ayyad, Tahani H.. Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 7, pp.1697-1703.
https://search.emarefa.net/detail/BIM-781636
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1703
Record ID
BIM-781636