Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method
Joint Authors
Khan, Muhammad Rashid
Qamar, Wajhul
Arafah, Azhar
Source
Saudi Journal of Biological Sciences
Issue
Vol. 24, Issue 7 (30 Nov. 2017), pp.1465-1469, 5 p.
Publisher
Publication Date
2017-11-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
In case of studies associated with human genetics, genomics, and pharmacogenetics the genomic DNA is extracted from the buccal cells, whole blood etc.
Several methods are exploited by the researchers to extract DNA from the whole blood.
One of these methods, which utilizes cell lysis and proteolytic properties of sodium dodecyl sulfate (SDS) and proteinase K respectively, might also be called SDS-PK method.
It does not include any hazardous chemicals such as phenol or chloroform and is inexpensive.
However, several researchers report the same method with different formulas and conditions.
During our experiments with whole blood DNA extraction we experienced problems such as protein contamination, DNA purity and yield when followed some SDS-PK protocols reported elsewhere.
A260/A280 and A260/A230 ratios along with PCR amplification give a clear idea about the procedure that was followed to extract the DNA.
In an effort to increase the DNA purity from human whole blood, we pointed out some steps of the protocol that play a crucial role in determining the extraction of high quality DNA.
American Psychological Association (APA)
Qamar, Wajhul& Khan, Muhammad Rashid& Arafah, Azhar. 2017. Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method. Saudi Journal of Biological Sciences،Vol. 24, no. 7, pp.1465-1469.
https://search.emarefa.net/detail/BIM-781652
Modern Language Association (MLA)
Qamar, Wajhul…[et al.]. Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method. Saudi Journal of Biological Sciences Vol. 24, no. 7 (Nov. 2017), pp.1465-1469.
https://search.emarefa.net/detail/BIM-781652
American Medical Association (AMA)
Qamar, Wajhul& Khan, Muhammad Rashid& Arafah, Azhar. Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method. Saudi Journal of Biological Sciences. 2017. Vol. 24, no. 7, pp.1465-1469.
https://search.emarefa.net/detail/BIM-781652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1469
Record ID
BIM-781652