Optimal conditions for bromelain extraction from pineapple fruit : ananas comosus
Joint Authors
al-Sadi, Ali J. R.
al-Hadban, Widian G. A.
al-Zubaydi, Maysa Adil Ali
Source
Engineering and Technology Journal
Issue
Vol. 34, Issue 5B (31 May. 2016), pp.675-682, 8 p.
Publisher
Publication Date
2016-05-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
Bromelain is a cysteine protease which is found in the tissues of Bromeliacea plant family of which pineapple Ananas cosmossus is best known.
The investigated parameters for optimal Brome-lain extraction are optimum buffers, pH, Molarity, time, and amounts of husk free pineapple fruit to volume (ml) of buffer ratio.
Sodium phosphate was best buffer for bromelain extraction from pine-apple fruit because its showed high activity, with casein as a substrate.
Subsequent experiments, using sodium phosphate as an optimal buffer for extraction and casein as a substrate, reviled that the optimal bromelain extraction conditions were achieved at pH 7.0, 0.1 M of sodium phosphate, 2.5 min of extraction time, 1:0.5 (gm of pineapple fruit/ v of sodium phosphate buffer) extraction per-centage, and 30 min of incubation time.
Additionally, Bromelain extracted from pineapple fruit showed a maximum enzyme activity at pH 7and at 30 min of incubation with casein as substrate.
American Psychological Association (APA)
al-Sadi, Ali J. R.& al-Hadban, Widian G. A.& al-Zubaydi, Maysa Adil Ali. 2016. Optimal conditions for bromelain extraction from pineapple fruit : ananas comosus. Engineering and Technology Journal،Vol. 34, no. 5B, pp.675-682.
https://search.emarefa.net/detail/BIM-783769
Modern Language Association (MLA)
al-Sadi, Ali J. R.…[et al.]. Optimal conditions for bromelain extraction from pineapple fruit : ananas comosus. Engineering and Technology Journal Vol. 34, no. 5B (2016), pp.675-682.
https://search.emarefa.net/detail/BIM-783769
American Medical Association (AMA)
al-Sadi, Ali J. R.& al-Hadban, Widian G. A.& al-Zubaydi, Maysa Adil Ali. Optimal conditions for bromelain extraction from pineapple fruit : ananas comosus. Engineering and Technology Journal. 2016. Vol. 34, no. 5B, pp.675-682.
https://search.emarefa.net/detail/BIM-783769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 681-682
Record ID
BIM-783769