![](/images/graphics-bg.png)
Isolation and Identification of toxigenic strains of E. coli from local cheese and evaluate their resistance to antibiotics
Other Title(s)
عزل و تشخيص سلالات E. coli المنتجة للسموم من الجبن المحلي و دراسة مقاومتها للمضادات الحياتية
Joint Authors
Jafar, Muhammad Musa
Kuraydi, Husayn Awdah
Khalid, Salwa khudadad
Abd, Inam Mahmud
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 7, Issue 1B (30 Jun. 2016), pp.199-205, 7 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Raw milk cheese is considered a srisk for food borne shiga-toxin-producing E.coli (STEC) contamination .The aim of the study is to isolation and identification of isolated strains by using serological and molecular methods.In this study 60 raw milk cheese samplas were collected from different local of Baghdad city (each 10 sample from each region of Baghdad) such as (Algadeda Baghdad,Gesser Diyala.Fudhaliyah.sader city.Kadhimiyh area and Rashid district)The isolated samples were cullured in selective media for identification and biochemical tests by using API 20 E and also by PCR technique for the presence of (STEC) by using bfp gene markers (stx1,stx2).
The results showed the presence of E.coli 0.26 haemorrhagic type (EHEC) and the Enteropathogenic strain of E.coli (0.86,0.127.0.128) and the Enteroaggregative strain of E.coli (0.55).
On the level of serological the isolates gave positive result that indicate presence of shiga toxin (stx1.stx2)in raw milk cheese .The result also showed multiple resistant of certain antibiotic(Amicillin 100%, Nalidixic acid and tetracycline and Gentamicin 80%, while was observed sensitive to Amikacin 100% and trimethoprim and cefotaxin 80.
American Psychological Association (APA)
Jafar, Muhammad Musa& Kuraydi, Husayn Awdah& Khalid, Salwa khudadad& Abd, Inam Mahmud. 2016. Isolation and Identification of toxigenic strains of E. coli from local cheese and evaluate their resistance to antibiotics. Kufa Journal for Veterinary Medical Sciences،Vol. 7, no. 1B, pp.199-205.
https://search.emarefa.net/detail/BIM-785266
Modern Language Association (MLA)
Jafar, Muhammad Musa…[et al.]. Isolation and Identification of toxigenic strains of E. coli from local cheese and evaluate their resistance to antibiotics. Kufa Journal for Veterinary Medical Sciences Vol. 7, no. 1B (2016), pp.199-205.
https://search.emarefa.net/detail/BIM-785266
American Medical Association (AMA)
Jafar, Muhammad Musa& Kuraydi, Husayn Awdah& Khalid, Salwa khudadad& Abd, Inam Mahmud. Isolation and Identification of toxigenic strains of E. coli from local cheese and evaluate their resistance to antibiotics. Kufa Journal for Veterinary Medical Sciences. 2016. Vol. 7, no. 1B, pp.199-205.
https://search.emarefa.net/detail/BIM-785266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 204-205
Record ID
BIM-785266