Essai biotechnologique de production d’acide glutamique par une souche de bacillus subtilis cultivees sur mout de rebuts de dattes (variete deglet Nour)‎

Abstract EN

The objective of this study is the production of glutamic acid by use of a strain of Bacillus subtilis B9 autochthon isolated from samples of sea water and using scrap dates variety deglet nour as fermentation medium.

And different culture conditions, the initial pH, the amount of inoculum, the sugar content, the content of glutamic acid, the source and the content of nitrogen were optimized.

Combination the results obtained show that the optimum amount of glutamic acid was obtained in an amount of 1 % inoculum was 38,84 g / L.

Moreover, a maximum quantity of glutamic acid was 38,84 g / L was obtained with an initial pH of 7.

Furthermore, an optimal amount of glutamic acid is 38.84 g / L was obtained by adding to the fermentation medium with a sugar content of 30 g / L.

In addition, a maximum production of glutamic acid is 55,28g / L was obtained by adding to the fermentation medium 5 g / L of glutamic acid.

On the other hand, a maximum production of glutamic acid is 65,76g/L was obtained by adding to the fermentation medium 10 g /L ammonium sulfate.

Finally, the optimum conditions for maximum production of glutamic acid are: amount of inoculum of 1.0 %, an initial pH of 7, a sugar content of 30 g / L, a glutamic acid content of 5 g / L, the use of ammonium sulfate as nitrogen source in an amount of 10 g / L.

An amount and a maximum yield of glutamic acid to be 65.76 g / L and 2.393 g/g of sugar, respectively were obtained with these optimal conditions.