The molecular and biological study of the isolated bacteriophages infecting pseudomonas aeruginosa from sewage water
Joint Authors
al-Saffar, Marwah Fadil
Jar Allah, Iman Muhammad
Source
Mesopotamia Environment Journal
Publisher
University of Babylon Environmental Research and Studies Center
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
19
Main Subjects
Natural & Life Sciences (Multidisciplinary)
English Abstract
Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms mostfrequently isolated worldwide.
And because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment.
Previously, P.
aeruginosa phages were isolatedbest candidates were chosen based on their ability to form clear plaques and their host range.
This work aimed to characterize one of those phages and evaluate the antibacterial activity of isolated bacteriophages in laboratory animal .
This studywas carried out during the period from June 2015 to January / 2016.
It included used of P.aeruginosa phage samples collected from sewage water.Fourtype of P.aeruginosaphage wereidentified according to genetic study, However, analysis of the P.
aeruginosa phages genome revealed that it was show closed related to NCBI-Blast Pseudomonas phage (KU297675.1) and others The local Pseudomonas phage isolates (F2 FF, E1 EF, and F1 FF) were show as unique isolates and different than NCBI-Blast Pseudomonas phage.
The results showed the vast diversity of P.
aeruginosaphages may be useful in treatment of infection with antibiotic resistant bacteria.We studied the biological activity of P.aeruginosa phage isolate in the burned Rabbits model.The result showedresponsible for a significant reduction in the bacterial population.
The burned mouse model showed an animal survival between 80 % and 100 %, significantly different from the control animals (0 %).All the rabbits completely recovered after 8 days of treatment and showed the absence of signs after 18 days after phage application in treatment.So, considered as a good candidate for phage-therapy.
Data Type
Conference Papers
Record ID
BIM-787075
American Psychological Association (APA)
al-Saffar, Marwah Fadil& Jar Allah, Iman Muhammad. 2017-12-31. The molecular and biological study of the isolated bacteriophages infecting pseudomonas aeruginosa from sewage water. National conference of science and art (1st : 2016 : University of Babylon, Iraq). . Vol. 3, Special Issue C (2017), pp.64-82.Babylon Iraq : University of Babylon Environmental Research and Studies Center.
https://search.emarefa.net/detail/BIM-787075
Modern Language Association (MLA)
al-Saffar, Marwah Fadil& Jar Allah, Iman Muhammad. The molecular and biological study of the isolated bacteriophages infecting pseudomonas aeruginosa from sewage water. . Babylon Iraq : University of Babylon Environmental Research and Studies Center. 2017-12-31.
https://search.emarefa.net/detail/BIM-787075
American Medical Association (AMA)
al-Saffar, Marwah Fadil& Jar Allah, Iman Muhammad. The molecular and biological study of the isolated bacteriophages infecting pseudomonas aeruginosa from sewage water. . National conference of science and art (1st : 2016 : University of Babylon, Iraq).
https://search.emarefa.net/detail/BIM-787075