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Fish eco-genotoxicology : comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution
Joint Authors
Mahbub, Shahid
Husayn, Bilal
Sultana, Tibah
Sultana, Salma
Masud, Muhammad Sharif
Ahmad, Zubayr
Source
Saudi Journal of Biological Sciences
Issue
Vol. 25, Issue 2 (28 Feb. 2018), pp.389-398, 10 p.
Publisher
Publication Date
2018-02-28
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Topics
Abstract EN
Owing to white meat production Labeo rohita have vast economic importance, but its population has been reduced drastically in River Chenab due to pollution.
Atomic absorption spectrophotometry showed a merciless toxicity level of Cd, Cu, Mn, Zn, Pb, Cr, Sn and Hg.
Comet assay results indicated significant (p < .05) DNA fragmentation in Labeo rohita as 42.21 ± 2.06 %, 31.26 ± 2.41 % and 21.84 ± 2.21 % DNA in comet tail, tail moment as 17.71 ± 1.79, 10.30 ± 1.78 and 7.81 ± 1.56, olive moment as 13.58 ± 1.306, 8.
10 ± 1.04 and 5.88 ± 0.06, respectively, from three different polluted sites on the river.
Micronucleus assay showed similar findings of single micronucleus induction (MN) as 50.00 ± 6.30 ‰, double MN 14.
40 ± 2.56 ‰ while nuclear abnormalities (NA) were found as 150.00 ± 2.92 ‰.
These higher frequencies, of MN induction and NA were found to be the cause of reduction of 96 % of the population of this fish species in an experimental area of the River Chenab.
This fish species has been found near extinction through the length of the river Chenab and few specimens in rainy seasons if restored by flood, may die in sugarcane mill season.
Due to sweeping extinction Labeo rohita showed the highest sensitivity for pollution and could be used as bioindicator and DNA fragmentation in this column feeder fish species as a biomarker of the pollution load in freshwater bodies.
American Psychological Association (APA)
Husayn, Bilal& Sultana, Tibah& Sultana, Salma& Masud, Muhammad Sharif& Ahmad, Zubayr& Mahbub, Shahid. 2018. Fish eco-genotoxicology : comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution. Saudi Journal of Biological Sciences،Vol. 25, no. 2, pp.389-398.
https://search.emarefa.net/detail/BIM-787342
Modern Language Association (MLA)
Husayn, Bilal…[et al.]. Fish eco-genotoxicology : comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution. Saudi Journal of Biological Sciences Vol. 25, no. 2 (Feb. 2018), pp.389-398.
https://search.emarefa.net/detail/BIM-787342
American Medical Association (AMA)
Husayn, Bilal& Sultana, Tibah& Sultana, Salma& Masud, Muhammad Sharif& Ahmad, Zubayr& Mahbub, Shahid. Fish eco-genotoxicology : comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution. Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 2, pp.389-398.
https://search.emarefa.net/detail/BIM-787342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 398
Record ID
BIM-787342