Spectrophotometric determination of progesterone and dopamine in breast cancer serum
Author
Source
Issue
Vol. 12, Issue 1 (31 Mar. 2017), pp.1-17, 17 p.
Publisher
University of Thi-Qar Research and Development Department
Publication Date
2017-03-31
Country of Publication
Iraq
No. of Pages
17
Main Subjects
Topics
Abstract EN
Dopamine (DA) is one of the most important catecholamine neurotransmitters in the human central nervous system in the brain and plays a key role in the functioning of the renal, hormonal, and cardiovascular systems.
Abnormal release of DA will contribute to some diseases such as Alzheimer’s and Parkinson's disease.
Therefore, the sensitive determination of DA becomes increasingly significant in the field of clinical disease diagnosis and the research of physiological functions.
Progesterone is an essential for the development and cyclical regulation of hormone responsive tissues including the breast and reproductive tract.
In the breast, progesterone acts in concert with estrogen to promote proliferative and pro-survival gene programs.
Progesterone has actions in breast cancer.
This paper shows a spectrophotometric method for determination dopamine and progesterone concentration in serum samples, fifty six veins blood samples collected from healthy control and breast cancer patient groups.
The analytical data obtained by using UVSpectrophotometer (max = 250, 266 nm), linearity (0.5 - 2.0, 0.25 - 1.5 ng/ml) for dopamine, progesterone respectively.
The molar absorptivity (), correlation coefficient (R2) and limit of detection (LOD) for dopamine, progesterone ( =2.5156 106, 4.1935 106 L.mol-1 cm-1), (R2= 0.9979, 0.99957) and (0.64, 0.57 ng/ml) respectively.
The results show significant differences between the concentrations of dopamine and progesterone in control and breast cancer patient groups (p0.05).It is fast, sensitive, selective and reliable quantification spectrophotometric method used in complex biological samples
American Psychological Association (APA)
Najm, Safa Sabri. 2017. Spectrophotometric determination of progesterone and dopamine in breast cancer serum. University of Thi-Qar Journal،Vol. 12, no. 1, pp.1-17.
https://search.emarefa.net/detail/BIM-787767
Modern Language Association (MLA)
Najm, Safa Sabri. Spectrophotometric determination of progesterone and dopamine in breast cancer serum. University of Thi-Qar Journal Vol. 12, no. 1 (Mar. 2017), pp.1-17.
https://search.emarefa.net/detail/BIM-787767
American Medical Association (AMA)
Najm, Safa Sabri. Spectrophotometric determination of progesterone and dopamine in breast cancer serum. University of Thi-Qar Journal. 2017. Vol. 12, no. 1, pp.1-17.
https://search.emarefa.net/detail/BIM-787767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 12-17
Record ID
BIM-787767