Production and extraction of siderophores-catecholate- from (MDR) Acinetobacter baumannii
Other Title(s)
إنتاج و استخلاص مركبات السايدروفور-نوع الكاتيكولات-من بكتريا Acinetobacter baumannii متعددة المقاومة للمضادات الحيوية
Author
Source
Issue
Vol. 14, Issue 2 (30 Jun. 2017), pp.356-362, 7 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Siderophores are low molecular weight organic compounds produced by microorganisms growing under low iron concentration.In this study we describe the detection, production and extraction of siderophores secreted by Acinetobacter baumannii (Multiple-drug resistant ) pathogens.
One hundered twenty Gram –negative non lactose fermenter bacilli isolates have been collected from three hospitals at Baghdad city over three months.
Primary identification of these isolates is performed by standard diagnostic methods (biochemical tests and API 20 NE); 19 clinical isolates of A.
baumannii are cultured on CHROMagar (highly selective medium for detection of MDR Acinetobacter) as well as diagnoses is documented by using Vitek 2 system.
Isolates are examined towards 11 different antibiotics.
High resistance is recognized for most isolates.
Detection of siderophore has been done by examining the isolates on M9 minimum medium; 5 isolates (26%) are producers for siderophore, the highest producing one is isolated from sputum and chosen to extract siderophore catecholate .
(Ab5S) isolate is examined on specific synthetic medium for production then siderophore molecules are extracted by ethyl acetate .Weight of dried extract is determined (115 mg/ml) and siderophore chemical nature has been assessed which appeared as catecholate.
American Psychological Association (APA)
Khalkhal, Intisar Nazim. 2017. Production and extraction of siderophores-catecholate- from (MDR) Acinetobacter baumannii. Baghdad Science Journal،Vol. 14, no. 2, pp.356-362.
https://search.emarefa.net/detail/BIM-790448
Modern Language Association (MLA)
Khalkhal, Intisar Nazim. Production and extraction of siderophores-catecholate- from (MDR) Acinetobacter baumannii. Baghdad Science Journal Vol. 14, no. 2 (Jun. 2017), pp.356-362.
https://search.emarefa.net/detail/BIM-790448
American Medical Association (AMA)
Khalkhal, Intisar Nazim. Production and extraction of siderophores-catecholate- from (MDR) Acinetobacter baumannii. Baghdad Science Journal. 2017. Vol. 14, no. 2, pp.356-362.
https://search.emarefa.net/detail/BIM-790448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 361-362
Record ID
BIM-790448